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Self-encapsulation and controlled release of recombinant proteins using novel silica-forming peptides as fusion linkers

Published on Mar 1, 2019in International Journal of Biological Macromolecules4.78
· DOI :10.1016/j.ijbiomac.2018.12.160
Mohamed A.A. Abdelhamid (Minia University), Ki Baek Yeo4
Estimated H-index: 4
(KU: Korea University)
+ 1 AuthorsSeung Pil Pack16
Estimated H-index: 16
(KU: Korea University)
Abstract
Abstract Recently, the potential use of biomimetic silica as smart matrices for the auto-encapsulation and controlled release of functional proteins has gained increased interest because of the mild synthesis conditions. Inspired by biological silicification, in this study, we studied novel silica-forming peptides (SFPs), Volp1 and Salp1, to mediate the generation of silica hybrids in vitro . The fusion of SFPs to model fluorescent proteins directed their auto-encapsulation in wet sol-gel silica materials. Furthermore, the SFPs served as affinity linkers for the immobilization of recombinant proteins in silica. Interestingly, the SFP fusion proteins modulated silicic acid polycondensation and allowed for the self-immobilization of SFP fusion proteins in two distinct silica formulations depending on the ionic strength—precipitated silica particles or wet silica gel. The controlled release of Salp1/Volp1 fusion proteins from silica matrices was significantly greater than that of the silaffin R5 fusion proteins. Subsequently, we showed that multiple SFP-tagged proteins homogenously entrapped within a silica matrix could be separately released following pre-incubation with different concentrations of l -arginine solution. These new findings provide a simple and reproducible route for silica hybrid formation for in situ stable auto-encapsulation and the sustained release of recombinant proteins with potential applications in biotechnology.
  • References (47)
  • Citations (1)
References47
Newest
#1Marion J. Limo (NTU: Nottingham Trent University)H-Index: 4
#2Anna Sola-Rabada (NTU: Nottingham Trent University)H-Index: 3
Last.Carole C. Perry (NTU: Nottingham Trent University)H-Index: 44
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#1Damian Pawolski (TUD: Dresden University of Technology)H-Index: 3
#2Christoph Heintze (TUD: Dresden University of Technology)H-Index: 1
Last.Nils Kröger (TUD: Dresden University of Technology)H-Index: 8
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#1Mi-Ran Ki (KU: Korea University)H-Index: 5
#2Thi Khoa My Nguyen (KU: Korea University)H-Index: 2
Last.Seung Pil Pack (KU: Korea University)H-Index: 16
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#1Alexander Kotzsch (TUD: Dresden University of Technology)H-Index: 13
#2Philip Gröger (TUD: Dresden University of Technology)H-Index: 4
Last.Nils Kröger (TUD: Dresden University of Technology)H-Index: 8
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#1Ki Baek Yeo (KU: Korea University)H-Index: 4
#2Mi-Ran Ki (KU: Korea University)H-Index: 5
Last.Seung Pil Pack (KU: Korea University)H-Index: 16
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#1Anne Drathen (Technical University of Dortmund)H-Index: 1
#2Jens Reiber (KIT: Karlsruhe Institute of Technology)H-Index: 2
Last.Susanne Brakmann (Technical University of Dortmund)H-Index: 4
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View next paperImmobilising proteins on silica with site-specifically attached modified silaffin peptides