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Dehydration stress responses of yeasts Torulaspora delbrueckii, Metschnikowia pulcherrima and Lachancea thermotolerans: Effects of glutathione and trehalose biosynthesis

Published on Jun 1, 2019in Food Microbiology 4.09
· DOI :10.1016/j.fm.2018.12.008
Abstract
Abstract In food industry and winemaking, the use of active dehydrated yeast (ADY) Saccharomyces cerevisiae is a frequent practice because of the long-term stability and high efficiency of ADY. Nowadays, there is an increasing interest for new yeasts strains, such as Torulaspora delbrueckii (Td), Metschnikowia pulcherrima (Mp) and Lachancea thermotolerans (Lt). However, the yeasts transformation processes into the solidified form generate several stresses that reduce the cell viability. In this case, understanding the phenomena of yeast cell resistance before, during and after dehydration is of great importance. In this study we analyzed two compounds associated with resistance to stress and produced by cells, glutathione (total, oxidized and reduced) and trehalose, at different stages of the process. The impact of growing and dehydration conditions on cell viability was analyzed by flow cytometry and two-photon laser scanning microscopy. The results showed that cells naturally enriched in glutathione or trehalose acquired resistance to dehydration, preventing the oxidation of glutathione in a growth/dehydration condition dependent manner. This is the first time that simultaneous metabolic and dehydration responses were observed in three non- Saccharomyces strains. These findings represent an opportunity to better understand the yeast's dehydration resistance phenomena and thus to promote the efficient industrial production of new dried yeasts.
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References69
Published on Apr 1, 1999in Biotechnology Letters 1.85
Petra Bafrncová2
Estimated H-index: 2
,
Daniela Šmogrovičová6
Estimated H-index: 6
+ 2 AuthorsZoltán Dömény6
Estimated H-index: 6
The final ethanol concentration achieved was increased by 17% (to 103 g ethanol/l) when excess assimilable nitrogen was added to the batch very high gravity (VHG) ethanolic fermentations by Saccharomyces cerevisiae. The supplementation of the media with 12 g yeast extract l−1, 0.3 g cell walls l−1, 3 g glycine l−1 and 20 g soya flour l−1 led to halving reduction of the fermentation time to 28 h. The ethanol productivity was enhanced by more than 50% (to achieved value 3.3 g l−1 h−1).
94 Citations Source Cite
Published on Jan 1, 2016in Lwt - Food Science and Technology 3.13
Dai Chen1
Estimated H-index: 1
(National University of Singapore),
Shao-Quan Liu28
Estimated H-index: 28
(National University of Singapore)
Abstract This study evaluated the effects of simultaneous and sequential fermentation with Torulaspora delbrueckii PRELUDE and Saccharomyces cerevisiae MERIT.ferm non-volatile and volatile components of on lychee wines. T. delbrueckii monoculture showed lower fermentative activity than S. cerevisiae alone with regard to growth, nitrogen utilization, production of alcohols, volatile acids and esters. However, T. delbrueckii monoculture had a better ability to retain the odour-active terpenes and ...
10 Citations Source Cite
Published on Sep 1, 1980in Journal of Bacteriology 3.22
S H Lillie1
Estimated H-index: 1
,
John R. Pringle68
Estimated H-index: 68
The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a general response to nutrient limitation. The patterns of accumulation and utilization of glycogen and trehalose were not identical under these conditions, suggesting that the two ca...
563 Citations
Published on Aug 1, 2015in Environmental Microbiology 4.97
Stéphane Guyot8
Estimated H-index: 8
,
Patrick Gervais37
Estimated H-index: 37
+ 3 AuthorsHazel M. Davey20
Estimated H-index: 20
(Aberystwyth University)
Environmental heat stress impacts on the physiology and viability of microbial cells with concomitant implications for microbial activity and diversity. Previously, it has been demonstrated that gradual heating of Saccharomyces cerevisiae induces a degree of thermal resistance, whereas a heat shock results in a high level of cell death. Here, we show that the impact of exogenous nutrients on acquisition of thermal resistance differs between strains. Using single-cell methods, we demonstrate the ...
20 Citations Source Cite
Published on May 1, 1990in Applied and Environmental Microbiology 3.63
A Hino1
Estimated H-index: 1
,
K Mihara1
Estimated H-index: 1
+ 1 AuthorsH Takano1
Estimated H-index: 1
Abstract Five freeze-tolerant yeast strains suitable for frozen dough were compared with ordinary commercial bakers' yeast. Kluyveromyces thermotolerans FRI 501 cells showed high survival ability after freezing when their resting cells were fermented for 0 to 180 min in modified liquid medium, and they grew to log and stationary phases. Among the freeze-tolerant strains of Saccharomyces cerevisiae, FRI 413 and FRI 869 showed higher surviving and trehalose-accumulating abilities than other S. cer...
109 Citations
Published on Sep 1, 2014in International Microbiology 0.80
López-Martínez G1
Estimated H-index: 1
,
Borrull A1
Estimated H-index: 1
+ 2 AuthorsRicardo Cordero-Otero6
Estimated H-index: 6
(Rovira i Virgili University)
In this study, we analyzed the metabolite features of the yeasts Saccharomyces cerevisiae, Naumovia castellii, and Saccharomyces mikatae. The three species are closely related genetically but differ in their tolerance of desiccation stress. Specifi cally, we determined whether certain metabolites correlated with cell viability after stress imposition. The metabolomic profi les of these strains were compared before cell desiccation and after cell rehydration. In S. mikatae, the presence of lysine...
5 Citations Source Cite
Published on Aug 1, 2015in Current Genetics 3.57
Elis C.A. Eleutherio15
Estimated H-index: 15
(Federal University of Rio de Janeiro),
Anita D. Panek30
Estimated H-index: 30
(Federal University of Rio de Janeiro)
+ 2 AuthorsRayne S. S. Magalhães2
Estimated H-index: 2
(Federal University of Rio de Janeiro)
Establishing the function of trehalose in yeast cells has led us, over the years, through a long path—from simple energy storage carbohydrate, then a stabilizer and protector of membranes and proteins, through a safety valve against damage caused by oxygen radicals, up to regulator of the glycolytic path. In addition, trehalose biosynthesis has been proposed as a target for novel drugs against several pathogens. Since this pathway is entirely absent in mammalian cells and makes use of highly spe...
44 Citations Source Cite
Published on May 2, 2007in World Journal of Microbiology & Biotechnology 2.10
K. Kapsopoulou1
Estimated H-index: 1
,
A. Mourtzini1
Estimated H-index: 1
+ 1 AuthorsE. Nerantzis1
Estimated H-index: 1
Four mixed culture fermentations of grape must were carried out with Kluyveromyces thermotolerans strain TH941 and Saccharomyces cerevisiae strain SCM952. In the first culture, both yeasts were added together, whereas in the remaining three cultures S. cerevisiae was added 1, 2, and 3 days after the inoculation of K. thermotolerans. The growth and survival of the K. thermotolerans strain and the amount of the produced l-lactic acid depend on the time of inoculation of the S. cerevisiae strain an...
52 Citations Source Cite
Published on Jun 1, 2014in Aging Cell 7.63
Jia Hu5
Estimated H-index: 5
(University of Southern California),
Min Wei26
Estimated H-index: 26
(University of Southern California)
+ 6 AuthorsValter D. Longo51
Estimated H-index: 51
(University of Southern California)
In mammals, extended periods of fasting leads to the accumulation of blood ketone bodies including acetoacetate. Here we show that similar to the conversion of leucine to acetoacetate in fasting mammals, starvation conditions induced ketone body-like acetic acid generation from leucine in S. cerevisiae. Whereas wild-type and ras2Δ cells accumulated acetic acid, long-lived tor1Δ and sch9Δ mutants rapidly depleted it through a mitochondrial acetate CoA transferase-dependent mechanism, which was es...
27 Citations Source Cite
Published on Dec 1, 2004in Enzyme and Microbial Technology 2.93
Shaohong Wen3
Estimated H-index: 3
(Beijing University of Chemical Technology),
Tao Zhang4
Estimated H-index: 4
(Beijing University of Chemical Technology),
Tianwei Tan44
Estimated H-index: 44
(Beijing University of Chemical Technology)
The effects of amino acids on glutathione (GSH) production by Saccharomyces cerevisiae T65 were investigated in this paper. Cysteine was the most important amino acids, which increased intracellular GSH content greatly but inhibited cell growth at the same time. The suitable amino acids addition strategy was two-step addition: in the first step, cysteine was added after two hours culture to 2 mM and then, the three amino acids (glutamic acid, glycine, and serine) were added after seven hours cul...
56 Citations Source Cite
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