Subcritical water extraction, identification and antiproliferation ability on HepG2 of polyphenols from lotus seed epicarp

In this study, polyphenols were extracted with subcritical water (SWE) from lotus seed epicarp. Based on single factor experiments, the extraction parameters were optimized by response surface methodology (RSM). Lotus seed epicarp polyphenols (LSEP) were purified by column chromatography (AB-8 macroporous resin) and identified by HPLC-ESI-MS. The antiproliferation ability of LSEP was also investigated. The optimal extraction parameters for the...