An LDHa single allele CHO cell mutant exhibits altered metabolic state and enhanced culture performance

BACKGROUND Reducing lactate production in animal cell cultures has been reported to improve cell culture performance and productivity of recombinant protein. A novel genome editing tool, CRISPR/Cas, has been used widely to induce double‐strand breaks in the genome and introduce targeted mutations efficiently. RESULTS In the present work, we used a publicly available human‐codon optimized CRISPR/Cas system to introduce mutations in one of the...