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Biotechnological applications of quorum sensing inhibition as novel therapeutic strategies for multidrug resistant pathogens

Published on Feb 1, 2019in Microbial Pathogenesis2.581
· DOI :10.1016/j.micpath.2018.11.043
Mona I. Shaaban14
Estimated H-index: 14
(Technology College),
Abdelaziz Elgaml5
Estimated H-index: 5
(Mansoura University),
El-Sayed E. Habib17
Estimated H-index: 17
(Technology College)
Abstract
Abstract High incidence of antibiotic resistance among bacterial clinical isolates necessitates the discovery of new targets for inhibition of microbial pathogenicity, without stimulation of microbial resistance. This could be achieved by targeting virulence determinants, which cause host damage and disease. Many pathogenic bacteria elaborate signaling molecules for cellular communication. This signaling system is named quorum sensing system (QS), and it is contingent on the bacterial population density and mediated by signal molecules called pheromones or autoinducers (AIs). Bacteria utilize QS to regulate activities and behaviors including competence, conjugation, symbiosis, virulence, motility, sporulation, antibiotic production, and biofilm formation. Hence, targeting bacterial communicating signals and suppression of QS exhibit a fundamental approach for competing microbial communication. In this review, we illustrate the common up to date approaches to utilize QS circuits in pathogenic bacteria, including Vibrio fischeri, Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumannii, as novel therapeutic targets.
  • References (97)
  • Citations (4)
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References97
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#1Jing Luo (Guangxi Medical University)H-Index: 2
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Last. Yiqiang Chen (Guangxi Medical University)H-Index: 9
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The quorum sensing (QS) circuit plays a role in the precise regulation of genes controlling virulence factors and biofilm formation in Pseudomonas aeruginosa. QS-controlled biofilm formation by Pseudomonas aeruginosa in clinical settings has remained controversial due to emerging drug resistance; therefore, screening diverse compounds for anti-biofilm or anti-QS activities is important. This study demonstrates the ability of sub-minimum inhibitory concentrations (sub-MICs) of baicalin, an active...
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#1Jon E. PaczkowskiH-Index: 3
#2Sampriti MukherjeeH-Index: 6
Last. Bonnie L. Bassler (HHMI: Howard Hughes Medical Institute)H-Index: 74
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Abstract Quorum sensing is a process of cell-cell communication that bacteria use to regulate collective behaviors. Quorum sensing depends on the production, detection, and group-wide response to extracellular signal molecules called autoinducers. In many bacterial species, quorum sensing controls virulence factor production. Thus, disrupting quorum sensing is considered a promising strategy to combat bacterial pathogenicity. Several members of a family of naturally-produced plant metabolites ca...
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#1Assia Guendouze (AMU: Aix-Marseille University)H-Index: 1
#2Laure PlenerH-Index: 5
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Pseudomonas aeruginosa is a Gram negative pathogenic bacteria involved in many human infections including otitis, keratitis, pneumonia and diabetic foot ulcers. P. aeruginosa uses a communication system, referred to as quorum sensing (QS), to adopt a group behaviour by synchronizing the expression of certain genes. Among the regulated traits, secretion of proteases or siderophores, motility and biofilm formation are mainly involved in the pathogenicity. Many efforts have been dedicated to the de...
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#1July Fong (NTU: Nanyang Technological University)H-Index: 3
#2Mingjun Yuan (NTU: Nanyang Technological University)H-Index: 4
Last. MichaelGIVSKOV (UCPH: University of Copenhagen)H-Index: 98
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Since its discovery 22 years ago, the bacterial cell-to-cell communication system, termed quorum sensing (QS), has shown potential as antipathogenic target. Previous studies reported that ajoene from garlic inhibits QS in opportunistic human pathogen Pseudomonas aeruginosa. In this study, screening of an in-house compound library revealed two sulfur-containing compounds which possess structural resemblance with ajoene and inhibit QS in bioreporter assay. Following a quantitative structure–activi...
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#1Abdelaziz Elgaml (Mansoura University)H-Index: 5
#2Shin-ichi Miyoshi (Okayama University)H-Index: 6
Vibrio vulnificus, a gram-negative halophilic estuarine bacterium, is an opportunistic human pathogen that causes rapidly progressive fatal septicemia and necrotizing wound infection. This species also causes hemorrhagic septicemia called vibriosis in cultured eels. It has been proposed that a range of virulence factors play roles in pathogenesis during human and/or eel infection. Among these factors, a metalloprotease (V. vulnificus protease [VVP]) and a cytolytic toxin (V. vulnificus hemolysin...
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#1Soha El-Shaer (Mansoura University)H-Index: 1
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The spread of multidrug-resistant Pseudomonas aeruginosa isolates constitutes a serious clinical challenge. Bacterial efflux machinery is a crucial mechanism of resistance among P. aeruginosa. Efflux inhibitors such as phenylalanine arginyl β-naphthylamide (PAβN) promote the bacterial susceptibility to antimicrobial agents. The pathogenesis of P. aeruginosa is coordinated via quorum sensing (QS). This study aims to find out the impact of efflux pump inhibitor, PAβN, on QS and virulence attribute...
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#1Felipe Alves de Almeida (UFV: Universidade Federal de Viçosa)H-Index: 4
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Abstract Quorum sensing is a cell-to-cell communication mechanism leading to differential gene expression in response to high population density. The autoinducer-1 (AI-1) type quorum sensing system is incomplete in Escherichia coli and Salmonella due to the lack of the AI-1 synthase (LuxI homolog) responsible for acyl homoserine lactone (AHL) synthesis. However, these bacteria encode the AHL receptor SdiA (a LuxR homolog) leading to gene regulation in response to AI-1 produced by other bacteria....
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#1El Shaymaa Abdel-Sattar (Okayama University)H-Index: 1
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Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding...
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