DNA Origami as Seeds for Promoting Protein Crystallization

This study reports the first experimental evidence of DNA origami as a seed resulting in the increase in probability of protein crystallization. Using the DNA origami constructed from long single-stranded M13 DNA scaffolds folded with short single-stranded DNA staples, it was found that the addition of the DNA origami in concentrations of 2–6 nM to mixtures of a well-characterized protein (catalase) solution (1.0–7.0 mg/mL) resulted in a higher...