Antigen‐specific CD8 T cells in cell cycle circulate in the blood after vaccination

Although clonal expansion is a hallmark of adaptive immunity, the location(s) where antigen‐responding T cells enter cell cycle and complete it have been poorly explored. This lack of knowledge stems partially from the limited experimental approaches available. By using Ki67 plus DNA staining and a novel strategy for flow cytometry analysis, we distinguished antigen‐specific CD8 T cells in G 0 , in G 1 and in S‐G 2 /M phases of cell cycle after...