Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
Abstract
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target sites. Current off-target discovery methods work using purified DNA or specific cellular models but are incapable of direct detection in vivo. We developed DISCOVER-Seq (discovery of in situ Cas off-targets and verification by sequencing), a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair...
Paper Details
Title
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
Published Date
Apr 19, 2019
Journal
Volume
364
Issue
6437
Pages
286 - 289
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