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Atomistic Details of Chymotrypsin Conformational Changes upon Adsorption on Silica

Published on Dec 10, 2018in ACS Biomaterials Science & Engineering 4.51
· DOI :10.1021/acsbiomaterials.8b00819
Nils Hildebrand3
Estimated H-index: 3
(University of Bremen),
Monika Michaelis2
Estimated H-index: 2
(University of Bremen)
+ 9 AuthorsLucio Colombi Ciacchi20
Estimated H-index: 20
(University of Bremen)
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Abstract
Adsorption of enzymes on solid surfaces may lead to conformational changes that reduce their catalytic conversion activity and are thus detrimental to the efficiency of biotechnology or biosensing applications. This work is a joint theoretical and experimental endeavor in which we identify and quantify the conformational changes that chymotrypsin undergoes when in contact with the surface of amorphous silica nanoparticles. For this purpose, we use circular dichroism spectroscopy, standard molecular dynamics, and advanced-sampling methods. Only the combination of these techniques allowed us to pinpoint a destabilization effect of silica on specific structural motifs of chymotrypsin. They are linked by the possibility of theoretically predicting CD spectra, allowing us to elucidate the source of the experimentally observed spectral changes. We find that chymotrypsin loses part of its helical content upon adsorption, with minor perturbation of its overall tertiary structure, associated with changes in the ar...
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