A high throughput lentivirus sieving assay identifies neutralization resistant Envelope sequences and predicts in vivo sieving

An effective prophylactic vaccine against human immunodeficiency virus (HIV) will likely require a potent antibody response that can neutralize the virus at the mucosal portal of entry. The elicitation of potent broadly-neutralizing anti-sera will be an iterative process, optimizing candidates that only block a fraction of potential viral strains. This effect, termed “sieving”, is evidence of a partially efficacious vaccine. Understanding the...