Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
Abstract
Mass spectrometry is the most used method for protein identification and quantification. Here we developed four protein extraction protocols precisely for mass spectrometry, and we compared with other ones already published. The best protocol developed by us consists on a simple extraction solution, a heat-shock step, and does not use protease inhibitor; moreover, it is the most efficient and uniform among replicates, besides to be safe, cheap...
Paper Details
Title
Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
Published Date
Feb 1, 2019
Journal
Volume
567
Pages
90 - 95
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