Match!

Stage-specific modulation of antimüllerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture

Published on Nov 1, 2018in Fertility and Sterility5.41
· DOI :10.1016/j.fertnstert.2018.07.006
Jing Xu13
Estimated H-index: 13
(Oregon National Primate Research Center),
Maralee S. Lawson10
Estimated H-index: 10
(Oregon National Primate Research Center)
+ 2 AuthorsFuhua Xu1
Estimated H-index: 1
(OHSU: Oregon Health & Science University)
Cite
Abstract
Objective To study whether follicular growth and oocyte maturation can be improved by antimullerian hormone (AMH) modulation at specific stages of follicular development. Design Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting National primate research center. Animal(s) Adult, female rhesus macaques ( Macaca mulatta ). Intervention(s) None. Main Outcome Measure(s) Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signaling, cumulus cell glycolysis, and oocyte quality. Result(s) Primary follicles formed "organoids" and developed to the antral stage in group culture. AMH exposure during the preantral stage increased organoid diameters. Oocytes from the AMH-treated organoids had greater diameters and matured to the metaphase II (MII) stage. Secondary follicles developed to the antral stage during individual culture. The AMH exposure during the preantral stage and AMH antibody treatment during the antral stage increased follicle diameters, vascular endothelial growth factor and follistatin production, differentiation factor 9 expression, and oocyte diameters. The MII oocytes from the AMH-modulated group developed to the morula stage after IVF, with one to the blastocyst stage. Conclusion(s) AMH supplementation at the preantral stage and depletion at the antral stage enhanced primate follicular development and oocyte competence in vitro. The improved embryonic development supports in vitro follicle maturation as a potential approach for fertility preservation.
  • References (40)
  • Citations (1)
Cite
References40
Newest
Published on Jun 1, 2018in Nature Medicine30.64
Brooke Tata3
Estimated H-index: 3
(French Institute of Health and Medical Research),
Nour El Houda Mimouni1
Estimated H-index: 1
(French Institute of Health and Medical Research)
+ 12 AuthorsTerhi Piltonen19
Estimated H-index: 19
(OUH: Oulu University Hospital)
Polycystic ovary syndrome (PCOS) is the main cause of female infertility worldwide and corresponds with a high degree of comorbidities and economic burden. How PCOS is passed on from one generation to the next is not clear, but it may be a developmental condition. Most women with PCOS exhibit higher levels of circulating luteinizing hormone, suggestive of heightened gonadotropin-releasing hormone (GnRH) release, and anti-Mullerian hormone (AMH) as compared to healthy women. Excess AMH in utero m...
Published on Mar 1, 2018in Molecular Human Reproduction3.40
Marie McLaughlin13
Estimated H-index: 13
(Edin.: University of Edinburgh),
David F. Albertini28
Estimated H-index: 28
+ 2 AuthorsEvelyn E. Telfer39
Estimated H-index: 39
(Edin.: University of Edinburgh)
Published on Feb 1, 2018in Biology of Reproduction2.96
Jing Xu13
Estimated H-index: 13
(Oregon National Primate Research Center),
Fuhua Xu10
Estimated H-index: 10
(OHSU: Oregon Health & Science University)
+ 6 AuthorsCecily V. Bishop14
Estimated H-index: 14
(Oregon National Primate Research Center)
Published on Oct 26, 2017in The New England Journal of Medicine70.67
Jacques Donnez79
Estimated H-index: 79
,
Marie-Madeleine Dolmans45
Estimated H-index: 45
There has been a sharp increase in the demand for fertility preservation. This review summarizes the indications and current options and describes new techniques and strategies, including those for women with newly diagnosed malignant disease.
Published on Feb 1, 2017in Journal of Endocrinology4.38
Ebrahim Asadi4
Estimated H-index: 4
(UT: University of Tehran),
Atefeh Najafi5
Estimated H-index: 5
(UT: University of Tehran)
+ 9 AuthorsFariba Khosravi2
Estimated H-index: 2
Abstract Ovarian tissue cryopreservation together with follicle culture provides a promising technique for fertility preservation in cancer patients. The study aimed to evaluate follicle parameters in a culture medium supplemented with VEGFA165 and/or fetuin. Vitrified-warmed ovarian cortical pieces were divided randomly into four culture groups consisting of basic culture medium (control), and the basic culture medium supplemented with VEGFA165, fetuin or both. After six days of culture, we eva...
Published on Dec 1, 2016in Journal of Assisted Reproduction and Genetics2.82
Jing Xu13
Estimated H-index: 13
(Oregon National Primate Research Center),
Fuhua Xu10
Estimated H-index: 10
(Oregon National Primate Research Center)
+ 3 AuthorsBetsy Ferguson22
Estimated H-index: 22
(Oregon National Primate Research Center)
Purpose The main goals of this study were to investigate the expression of anti-Mullerian hormone (AMH) and its receptor (AMHR2) during follicular development in primates, and to evaluate the potential of AMH as a biomarker for follicle growth and oocyte maturation in vitro.
Published on Jul 1, 2016in Human Reproduction5.51
Jing Xu13
Estimated H-index: 13
(Oregon National Primate Research Center),
Cecily V. Bishop14
Estimated H-index: 14
(Oregon National Primate Research Center)
+ 2 AuthorsFuhua Xu10
Estimated H-index: 10
(Oregon National Primate Research Center)
What are the direct effects and physiological role of anti-Mullerian hormone (AMH) during primate follicular development and function at specific stages of folliculogenesis?AMH actions in the primate ovary may be stage-dependent, directly promoting pre-antral follicle growth while inhibiting antral follicle maturation and dominant follicle selection.AMH is expressed in the adult ovary, particularly in developing follicles. Studies in mice suggest that AMH suppresses pre-antral follicle growth in...
Published on Jun 1, 2016in Molecular Human Reproduction3.40
Saenz-de-Juano1
Estimated H-index: 1
,
Katy Billooye2
Estimated H-index: 2
+ 1 AuthorsEllen Anckaert20
Estimated H-index: 20
Does in vitro follicle culture (IFC) have an effect on maintenance of imprinted DNA methylation in preimplantation mouse embryos?We report similar alterations in the methylation pattern of H19 imprinted maternally expressed transcript (H19), small nuclear ribonucleoprotein polypeptide N (Snrpn) and mesoderm specific transcript (Mest) imprinted genes in mouse blastocysts obtained after ovulation induction and IFC. Furthermore, we observed no differences in the gene expression of maternal effect p...
Published on Apr 1, 2016in Nature Communications11.88
Irene Cimino5
Estimated H-index: 5
,
Filippo Casoni7
Estimated H-index: 7
+ 13 AuthorsDidier Dewailly47
Estimated H-index: 47
Anti-Mullerian hormone (AMH) plays a role in sexual differentiation and gonadal function, but extra-gonadal effects of AMH are not known. Here Cimino et al. show that AMH activates a subset of gonadotrophin-releasing hormone (GnRH)-releasing neurons, contributing to luteinizing hormone secretion from the pituitary gland.