Generation of PDX ‐1 mutant porcine blastocysts by introducing CRISPR /Cas9‐system into porcine zygotes via electroporation

Recently, we established the GEEP (“gene editing by electroporation of Cas9 protein”) method, in which the CRISPR /Cas9 system, consisting of a Cas9 protein and single guide RNA (sg RNA ), is introduced into pig zygotes by electroporation and thus induces highly efficient targeted gene disruption. In this study, we examined the effects of sg RNA on the blastocyst formation of porcine embryos and evaluated their genome‐editing efficiency. To...