Match!

Structure of the Ambrosia Beetle (Coleoptera: Curculionidae) Mycangia Revealed Through Micro-Computed Tomography.

Published on Sep 1, 2018in Journal of Insect Science1.446
· DOI :10.1093/JISESA/IEY096
You Li7
Estimated H-index: 7
(UF: University of Florida),
Yongying Ruan4
Estimated H-index: 4
(SZPT: Shenzhen Polytechnic)
+ 5 AuthorsJiri Hulcr27
Estimated H-index: 27
(UF: University of Florida)
Sources
Abstract
: Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae and Platypodinae) rely on a symbiosis with fungi for their nutrition. Symbiotic fungi are preserved and transported in specialized storage structures called mycangia. Although pivotal in the symbiosis, mycangia have been notoriously difficult to study, given their minute size and membranous structure. We compared the application of novel visualization methods for the study of mycangia, namely micro-computed tomography (micro-CT) and laser ablation tomography (LATscan) with traditional paraffin sectioning. Micro-CT scanning has shown the greatest promise in new organ discovery, while sectioning remains the only method with sufficient resolution for cellular visualization. All three common types of mycangia (oral, mesonotal, and pronotal) were successfully visualized and presented for different species of ambrosia beetles: Ambrosiodmus minor (Stebbing) 1909, Euplatypus compositus (Say) 1823, Premnobius cavipennis Eichhoff 1878, Scolytoplatypus raja Blandford 1893, Xylosandrus crassiusculus (Motschulsky) 1866 and X. amputatus (Blandford) 1894. A reconstruction of the mycangium and the surrounding musculature in X. amputatus is also presented. The advantages of micro-CT compared to the previously commonly used microtome sectioning include the easy visualization and recording of three-dimensional structures, their position in reference to other internal structures, the ability to distinguish natural aberrations from technical artifacts, and the unprecedented visualizations of the anatomic context of mycangia enabled by the integrated software.
  • References (54)
  • Citations (4)
📖 Papers frequently viewed together
14 Citations
10 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References54
Newest
#1You Li (UF: University of Florida)H-Index: 7
#2Yongying Ruan (SZPT: Shenzhen Polytechnic)H-Index: 4
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 5 authors...
Insects that depend on microbial mutualists evolved a variety of organs to transport the microsymbionts while dispersing. The ontogeny and variability of such organs is rarely studied, and the microsymbiont's effects on the animal tissue development remain unknown in most cases. Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae or Platypodinae) and their mutualistic fungi are an ideal system to study the animal–fungus interactions. While the interspecific diversity of their fungus transpor...
7 CitationsSource
#1Andrew J. Johnson (UF: University of Florida)H-Index: 4
#2Duane D. McKenna (U of M: University of Memphis)H-Index: 19
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 8 authors...
Abstract Bark and ambrosia beetles (Curculionidae, Scolytinae) display a conspicuous diversity of unusual genetic and ecological attributes and behaviors. Reconstructing the evolution of Scolytinae, particularly the large and ecologically significant tribe Cryphalini (pygmy borers), has long been problematic. These challenges have not adequately been addressed using morphological characters, and previous research has used only DNA sequence data from small numbers of genes. Through a combination ...
13 CitationsSource
#1Demian F. Gomez (UF: University of Florida)H-Index: 5
#2Robert J. Rabaglia (USFS: United States Forest Service)H-Index: 12
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 4 authors...
: Bark and ambrosia beetles (Scolytinae) are the most successful group of invasive wood borers worldwide, and the most invasive among them are species in the tribe Xyleborini. This haplodiploid, highly inbred, fungus-farming group is represented by 30 non-native species in North America, of which at least five are serious pests. The few identification resources for Xyleborini that exist are becoming outdated due to new species arrivals and nomenclatural changes. Here we present a new comprehensi...
21 CitationsSource
#1Craig Bateman (UF: University of Florida)H-Index: 11
#2Yin-Tse Huang (UF: University of Florida)H-Index: 4
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 6 authors...
Abstract Ambrosia beetles and fungi represent an interesting and economically important symbiosis, but the vast majority of ambrosia fungi remain unexplored, hindering research, management of pathogens, and mitigation of invasive species. Beetles in the subtribe Premnobiini are one example of an entire beetle lineage whose fungal symbionts have never been studied. Here, we identify one dominant fungal symbiont of Premnobius cavipennis by using fungus culturing, community sequencing, microtome se...
13 CitationsSource
#1Jiri Hulcr (UF: University of Florida)H-Index: 27
#2Lukasz L. Stelinski (UF: University of Florida)H-Index: 39
The ambrosia beetle–fungus farming symbiosis is more heterogeneous than previously thought. There is not one but many ambrosia symbioses. Beetle-fungus specificity is clade dependent and ranges from strict to promiscuous. Each new origin has evolved a new mycangium. The most common relationship with host trees is colonization of freshly dead tissues, but there are also parasites of living trees, vectors of pathogenic fungi, and beetles living in rotten trees with a wood-decay symbiont. Most of t...
76 CitationsSource
#1Matthew T. Kasson (WVU: West Virginia University)H-Index: 14
#2Kristen L. Wickert (WVU: West Virginia University)H-Index: 4
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 9 authors...
Most wood-boring insects compete with wood decaying basidiomycetes for woody biomass. One clade of ambrosia beetles gained access to rotten wood – an abundant resource unsuitable to most wood-boring insects – by evolving a farming-like mutualism with a white rot polypore. Here we show the mutualist of Ambrosiodmus/Ambrosiophilus, the polypore Flavodon ambrosius, is superior in lignocellulolytic capacity compared to Ascomycota ambrosia fungi and other white rot Basidiomycota. This mutualism facil...
21 CitationsSource
#1Yongying Ruan (CAS: Chinese Academy of Sciences)H-Index: 4
#2Dan Dan (CAS: Chinese Academy of Sciences)H-Index: 13
Last. Xing-Ke Yang (CAS: Chinese Academy of Sciences)H-Index: 15
view all 7 authors...
Background Innovative new techniques that aid in the visualization of microscopic anatomical structures have improved our understanding of organismal biology significantly. It is often challenging to observe internal 3D structures, despite the use of techniques such as confocal laser scanning microscopy (CLSM), micro-computed tomography (Micro-CT), magnetic resonance imaging (MRI), focused ion beam scanning electron microscopy (FIB-SEM) and others. In the current paper, we assess LED-SIM (DMD-ba...
3 CitationsSource
#1Verónica Quirici (Andrés Bello National University)H-Index: 11
#2Claudia Jimena Guerrero (Universidad Santo Tomás)H-Index: 3
Last. Rodrigo A. Vfisquez (University of Chile)H-Index: 26
view all 5 authors...
Background Environmental stressors increase the secretion of glucocorticoids that in turn can shorten telomeres via oxidative damage. Modification of telomere length, as a result of adversity faced early in life, can modify an individual’s phenotype. Studies in captivity have suggested a relationship between glucocorticoids and telomere length in developing individuals, however less is known about that relationship in natural populations.
79 CitationsSource
#1You Li (UF: University of Florida)H-Index: 7
#1Li You (UF: University of Florida)H-Index: 1
Last. Jiri Hulcr (UF: University of Florida)H-Index: 27
view all 7 authors...
Ambrosia symbiosis is an obligate, farming-like mutualism between wood-boring beetles and fungi. It evolved at least 11 times and includes many notorious invasive pests. All ambrosia beetles studied to date cultivate ascomycotan fungi: early colonizers of recently killed trees with poor wood digestion. Beetles in the widespread genus Ambrosiodmus, however, colonize decayed wood. We characterized the mycosymbionts of three Ambrosiodmus species using quantitative culturing, high-throughput metabar...
29 CitationsSource
#1Lucy A. WaltonH-Index: 5
#2Robert S. BradleyH-Index: 23
Last. Michael J. Sherratt (University of Manchester)H-Index: 31
view all 7 authors...
Characterisation and quantification of tissue structures is limited by sectioning-induced artefacts and by the difficulties of visualising and segmenting 3D volumes. Here we demonstrate that, even in the absence of X-ray contrast agents, X-ray computed microtomography (microCT) and nanotomography (nanoCT) can circumvent these problems by rapidly resolving compositionally discrete 3D tissue regions (such as the collagen-rich adventitia and elastin-rich lamellae in intact rat arteries) which in tu...
56 CitationsSource
Cited By4
Newest
#1Ignacio Alba-Alejandre (UGR: University of Granada)H-Index: 2
#2Javier Alba-Tercedor (UGR: University of Granada)H-Index: 17
Last. Wayne B. Hunter (ARS: Agricultural Research Service)H-Index: 31
view all 3 authors...
Huanglongbing (HLB) (citrus greening disease) is one of the most serious bacterial diseases of citrus. It is caused by (1) Candidatus Liberibacter africanus, transmitted by Trioza erytreae and (2) C.L. asiaticus and C.L. americanus, transmitted by Diaphorina citri. As part of a multidisciplinary project on D. citri (www.citrusgreening.org), we made a detailed study, using micro-computed tomography, of the female abdominal terminalia, reproductive system (ovaries, accessory glands, spermatheca, c...
Source
#1William R. Morrison (ARS: Agricultural Research Service)H-Index: 13
#2Asheesh Lanba (USM: University of Southern Maine)
Last. Alexander Bruce (ARS: Agricultural Research Service)H-Index: 2
view all 4 authors...
Abstract In the last century, there have been important advances in peering inside the internal grain environment. This has included electrical conductance mills, acoustical tools, near-infrared spectroscopy, and soft X-ray imaging. Each technique has their own advantages and limitations. In this current study, we describe a novel process for assessing grain quality and internal insect development in grain using laser ablation tomography (LATscan) and compare it to soft X-ray imaging for three l...
Source
#1Ignacio Alba-Alejandre (UGR: University of Granada)H-Index: 2
#2Javier Alba-Tercedor (UGR: University of Granada)H-Index: 17
Last. Fernando E. Vega (USDA: United States Department of Agriculture)H-Index: 37
view all 3 authors...
Traditionally, the study of anatomy in insects has been based on dissection techniques. Micro-computed tomography (micro-CT) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-CT scans to study, in detail, the internal structures and organs of the coffee berry borer (Hypothenemus hampei), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the...
2 CitationsSource
#1Zi‐Ru Jiang (Nagoya University)H-Index: 1
#2Shun'ichi Kinoshita (University of Vienna)H-Index: 1
Last. Hisashi Kajimura (Nagoya University)H-Index: 11
view all 5 authors...
2 CitationsSource