Defining CRISPR–Cas9 genome-wide nuclease activities with CIRCLE-seq

Volume: 13, Issue: 11, Pages: 2615 - 2642
Published: Oct 19, 2018
Abstract
Circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) is a sensitive and unbiased method for defining the genome-wide activity (on-target and off-target) of CRISPR-Cas9 nucleases by selective sequencing of nuclease-cleaved genomic DNA (gDNA). Here, we describe a detailed experimental and analytical protocol for CIRCLE-seq. The principle of our method is to generate a library of circularized gDNA with minimized...
Paper Details
Title
Defining CRISPR–Cas9 genome-wide nuclease activities with CIRCLE-seq
Published Date
Oct 19, 2018
Volume
13
Issue
11
Pages
2615 - 2642
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