Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma

Background: Extracellular vesicles (EVs) isolated from blood plasma are extensively investigated both as biomarkers and therapeutics. However, efficient isolation of high-purity EVs from blood plasma in quantities sufficient for in-vivo experiments is a great challenge. Thus, the aim of this study was to develop an isolation method to separate the majority of EVs from major impurities such as lipoprotein particles and abundant plasma proteins...