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Publisher Correction: Base editing: precision chemistry on the genome and transcriptome of living cells

Published on Dec 1, 2018in Nature Reviews Genetics43.704
· DOI :10.1038/s41576-018-0068-0
Holly A. Rees10
Estimated H-index: 10
(Broad Institute),
David R. Liu73
Estimated H-index: 73
(Broad Institute)
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Abstract
The originally published article contained errors in reference numbering throughout table 1 (DNA base editors and their approximate editing windows) due to the unintended propagation of reference numbering from an earlier version of the table. The article has now been corrected online. The editors apologize for this error.
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  • Citations (3)
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#1Gibran Ali (University of Health Sciences Lahore)
#2Muhammad Akram Tariq (University of Health Sciences Lahore)
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Beta (β)-thalassemia is one of the most significant hemoglobinopathy worldwide. The high prevalence of the β-thalassemia carriers aggravates the disease burden for patients and national economies in the developing world. The survival of β-thalassemia patients solely relies on repeated transfusions, which eventually results into multi-organ damage. The fetal γ-globin genes are ordinarily silenced at birth and replaced by the adult β-globin genes. However, mutations that cause lifelong persistence...
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#1Nicole M. GaudelliH-Index: 6
#2Dieter K. LamH-Index: 1
Last. Alexander J. LiquoriH-Index: 1
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The foundational adenine base editors (e.g. ABE7.10) enable programmable C:G to T:A point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosine deaminase variants to create ABE8s. At NGG PAM sites, ABE8s result in ~1.5x higher editing at protospacer positions A5-A7 and ~3.2x higher editing at positions A3-A4 and A8-A10 compared with ABE7.10. Non-NGG PAM variants have a ~4.2-fold overall higher on-targ...
1 CitationsSource
#1Erwei ZuoH-Index: 6
#2Yidi Sun (CAS: Chinese Academy of Sciences)H-Index: 8
Last. LIYixue (CAS: Chinese Academy of Sciences)H-Index: 57
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Base editors hold promise for correcting pathogenic mutations, while substantial single nucleotide variations (SNVs) on both DNA and RNA were generated by cytosine base editors (CBEs). Here we examined possibilities to reduce off-target effects by engineering cytosine deaminases. By screening 24 CBEs harboring various rAPOBEC1 (BE3) or human APOBEC3A (BE3-hA3A) mutations on the ssDNA or RNA binding domain, we found 8 CBE variations could maintain high on-target editing efficiency. Using Genome-w...
2 CitationsSource
#1Holly A. Rees (Broad Institute)H-Index: 10
#2Christopher Wilson (Broad Institute)H-Index: 3
Last. David R. Liu (Broad Institute)H-Index: 73
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Adenine base editors (ABEs) enable precise and efficient conversion of target A∙T base pairs to G·C base pairs in genomic DNA with a minimum of by-products. While ABEs have been reported to exhibit minimal off-target DNA editing, off-target editing of cellular RNA by ABEs has not been examined in depth. Here, we demonstrate that a current ABE generates low but detectable levels of widespread adenosine-to-inosine editing in cellular RNAs. Using structure-guided principles to design mutations in b...
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#1Ya-Ju Chang (Columbia University)
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#1Hui YangH-Index: 19
#2LIYixueH-Index: 57
Last. Lars M. Steinmetz (Stanford University)H-Index: 56
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Genome editing tools including CRISPR/Cas9 and base editors hold great promise for correcting pathogenic mutations. Unbiased genome-wide off-target effects of the editing in mammalian cells is required before clinical applications, but determination of the extent of off-target effects has been difficult due to the existence of single nucleotide polymorphisms (SNPs) in individuals. Here, we developed a method named GOTI (Genome-wide Off-target analysis by Two-cell embryo Injection) to detect off-...
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