A unified GenomeSpace recipe to identify essential genes and associated subnetworks from Genome-Scale CRISPR-Cas9 knockout screens

Daniel E. Carlin; Forrest Kim; Trey Ideker; Jill P. Mesirov

doi:https://doi.org/10.12688/f1000research.16290.1

doi.org/10.12688/f1000research.16290.1

A unified GenomeSpace recipe to identify essential genes and associated subnetworks from Genome-Scale CRISPR-Cas9 knockout screens

,

..., Jill P. Mesirov

Volume: 7, Pages: 1636 - 1636

Published: Oct 12, 2018

Abstract

We present a unified GenomeSpace recipe that combines the results of a high throughput CRISPR genetic screen and a biological network to return a subnetwork that suggests a mechanistic explanation of the screen’s results. The explanatory subnetwork is found by network propagation, a popular systems biology approach. We demonstrate our pipeline on an alpha toxin screen, revealing a subnetwork that is both highly interconnected and highly...

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Paper Details

Title

A unified GenomeSpace recipe to identify essential genes and associated subnetworks from Genome-Scale CRISPR-Cas9 knockout screens

DOI

doi.org/10.12688/f1000research.16290.1

Published Date

Oct 12, 2018

Journal

F1000Research

Volume

7

Pages

1636 - 1636

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