Effects of solution behavior on polysaccharide structure and inhibitory of α-glucosidase activity from Cordyceps militaris

Crude polysaccharide of Cordyceps militaris was obtained by hot water extraction, Sevag deproteinization and alcohol precipitation, and then purified by Sephadex G-200 column chromatography. The extraction purified was named as CPS. The effects of pH, Ca2+ and DMSO on the structure of the CPS were analyzed by HPLC, Congo red assay, circular dichroism spectra and Atomic force microscopy (AFM) respectively. The results showed that the great...