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High Fat Diet-Induced miR-122 Regulates Lipid Metabolism and Fat Deposition in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus) Liver

Published on Oct 5, 2018in Frontiers in Physiology3.201
· DOI :10.3389/fphys.2018.01422
Jun Qiang4
Estimated H-index: 4
(Chinese Academy of Fishery Sciences),
Yi Fan Tao2
Estimated H-index: 2
(NAU: Nanjing Agricultural University)
+ 4 AuthorsPao Xu9
Estimated H-index: 9
(Chinese Academy of Fishery Sciences)
Abstract
The liver is an important organ for the regulation of lipid metabolism. In genetically improved farmed tilapia (GIFT, Oreochromis niloticus), fat deposition in the liver occurs when they are fed high-lipid diets over a long term. This can affect their growth, meat quality, and disease resistance. MicroRNAs (miRNAs)are known to be crucial regulatory factors involved in lipid metabolism; however, the mechanism by which they regulate lipid deposition in GIFT remains unclear. Comparative miRNA expression profiling between GIFT fed a normal diet and those fed a high-lipid diet showed that miR-122 is closely related to lipid deposition. Using miR-122 as a candidate, we searched for a binding site for miR-122 in the 3′-untranslated region (UTR) of the stearoyl-CoA desaturase gene (SCD)using bioinformatics tools, and then confirmed its functionality using the luciferase reporter gene system. Then, the regulatory relationship between this miRNA and its target gene SCD was analyzed using real-time polymerase chain reaction (qRT-PCR) and western blotting analyses. Last, we investigated the effect of the loss of miR-122 expression on lipid metabolism in GIFT. The results showed that a sequence in the 3′-UTR region of SCD of GIFT was complementary to the miR-122 seed region, and there was a negative relationship between the expression of miRNA and SCD expression. Inhibition of miR-122 up-regulated SCD, increased the expression of fat synthesis-related genes, increased hepatic triglyceride and cholesterol contents, and promoted weight gain in fish. Our results showed that miR-122 targets SCD to mediate hepatic fat metabolism. These results provide new insights for the prevention and treatment of fatty liver disease in GIFT.
  • References (36)
  • Citations (2)
References36
Newest
#1Yi-Fan Tao (Chinese Academy of Fishery Sciences)H-Index: 6
#2Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
Last. Jing-Wen Bao (Chinese Academy of Fishery Sciences)H-Index: 3
view all 6 authors...
Abstract MicroRNAs (miRNAs) play vital roles in modulating diverse metabolic processes in the liver, including lipid metabolism. Genetically improved farmed tilapia (GIFT, Oreochromis niloticus ), an important aquaculture species in China, is susceptible to hepatic steatosis when reared in intensive culture systems. To investigate the miRNAs involved in GIFT lipid metabolism, two hepatic small RNA libraries from high-fat diet-fed and normal-fat diet-fed GIFT were constructed and sequenced using ...
6 CitationsSource
#1Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
#2Yi-Fan Tao (NAU: Nanjing Agricultural University)H-Index: 6
Last. Yi-Lan Sun (Chinese Academy of Fishery Sciences)H-Index: 5
view all 8 authors...
Darkbarbel catfish (Pelteobagrus vachellii) is an important freshwater fish in China. Water temperature greatly influences the absorption and utilization of dietary lipid by fish. Response values (including growth, hepatic fat deposition, and gene expression) for darkbarbel catfish mediated by two factors (water temperature 20–34 °C; dietary lipid level 2–17%) were the focus of this study. The relationship between the two factors and the response values was evaluated by the response surface meth...
7 CitationsSource
#1Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
#2Jie He (Chinese Academy of Fishery Sciences)H-Index: 9
Last. Zhixiang Zhu (Chinese Academy of Fishery Sciences)H-Index: 2
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The present study was performed to investigate the effects of dietary lipid level on growth performance, antioxidant defense, and mRNA levels of digestive enzymes and immune genes in larval genetically improved farmed tilapia (Oreochromis niloticus). The results show that larvae fed lipid levels of 4–11.5% had greater weight gain (WG), feed efficiency, protein efficiency ratio and survival than larvae fed the other dietary lipid levels (P 0.05) but lipid contents increased as dietary lipid level...
11 CitationsSource
#1Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
#2Yi Fan Tao (Chinese Academy of Fishery Sciences)H-Index: 1
Last. Pao Xu (Chinese Academy of Fishery Sciences)H-Index: 9
view all 5 authors...
ABSTRACT MicroRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression by binding to the 3′ untranslated region (3′ UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver biology and disease. Here, we report for the first time that miR-29a post-transcriptionally regulates stearoyl-CoA desaturase (SCD) by binding to its 3′ UTR in genetically improved farmed tilapia (GIFT), Oreochromis niloticus, as shown by a 3′ UTR luciferase repo...
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#1Xiao Xia Wang (Sichuan University)H-Index: 2
#2Ting Ye (Sichuan University)H-Index: 2
Last. Rui Liu (Sichuan University)H-Index: 9
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Reduced hepatic glycogenesis is one of the most important causes of metabolic abnormalities in non‑alcoholic fatty liver disease. Octreotide, a somatostatin analogue, has been demonstrated to promote weight loss and improve metabolic disorders in mice with high fat diet (HFD)‑induced obesity. However, whether octreotide affects hepatic glycogenesis is unknown. The aim of the present study was to verify the effects of octreotide on hepatic glycogenesis in rats with HFD‑induced obesity. Male Sprag...
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#1Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
#2Yi-Fan Tao (NAU: Nanjing Agricultural University)H-Index: 6
Last. Yi-Lan Sun (Chinese Academy of Fishery Sciences)H-Index: 5
view all 6 authors...
Abastract MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene expression by binding to the 3′untranslated region (3′UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver homeostasis and energy metabolism. Down-regulated levels of hepatic miR-122 were found in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to cadmium (Cd) stress. Here, we report for the first time that reduction of miR-122 post-transcrip...
20 CitationsSource
#1H. Giral (Charité)H-Index: 2
#2Adelheid Kratzer (Charité)H-Index: 4
Last. Ulf Landmesser (Charité)H-Index: 74
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Numerous studies have examined the role of microRNAs (miRNAs) in cell homeostasis and cardiovascular disease and have markedly improved our understanding of RNA biology in general and the potential role of miRNAs in atherosclerosis. In atherosclerosis, several miRNAs, such as miR-33a,b, miR-92a, miR-126 and others, have been identified that are relevant mediators of pathological processes, including regulation of cholesterol and lipid biosynthesis, lipoprotein metabolism and cholesterol efflux, ...
14 CitationsSource
#1Jianwen He (Chinese Academy of Fishery Sciences)H-Index: 2
#2Jun Qiang (Chinese Academy of Fishery Sciences)H-Index: 7
Last. R.Q. Yang (NAU: Nanjing Agricultural University)H-Index: 1
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Abstract We evaluated the effect of cold shock on the fatty acid composition, antioxidant enzymes, and physiological responses of genetically improved farmed tilapia (GIFT). Experimental GIFT tilapia, a warm-water teleost, were initially acclimated at 28 °C and then transferred directly to 13 °C. Stress responses were monitored for 120 h. There was a significant change in all parameters in response to the cold stressor ( P P
24 CitationsSource
#1Simonetta Bandiera (UDS: University of Strasbourg)H-Index: 6
#2Sébastien Pfeffer (UDS: University of Strasbourg)H-Index: 31
Last. Mirjam B. Zeisel (UDS: University of Strasbourg)H-Index: 37
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Summary Being the largest internal organ of the human body with the unique ability of self-regeneration, the liver is involved in a wide variety of vital functions that require highly orchestrated and controlled biochemical processes. Increasing evidence suggests that microRNAs (miRNAs) are essential for the regulation of liver development, regeneration and metabolic functions. Hence, alterations in intrahepatic miRNA networks have been associated with liver disease including hepatitis, steatosi...
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#1Camila G. Dossi (University of Chile)H-Index: 5
#2Gladys Tapia (University of Chile)H-Index: 24
Last. Amanda D'Espessailles (University of Chile)H-Index: 6
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Abstract Nonalcoholic fatty liver disease is characterized by an abnormal accumulation of triacylglycerides in the liver in absence of significant alcohol consumption. Under these conditions, it has been observed an impaired bioavailability of hepatic n-3 long-chain polyunsaturated fatty acids (LCPUFAs). The aim of this study was to test the reversion of the prosteatotic and proinflammatory effects of high-fat diet (HFD) in the mouse liver by changing to normocaloric diet and n-3 LCPUFA suppleme...
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Abstract Fatty liver injury (or disease) is a common disease in farmed fish, but its pathogenic mechanism is not fully understood. Therefore the present study aims to investigate high-fat diet (HFD)-induced liver injury and explore the underlying mechanism in fish. The tilapia were fed on control diet and HFD for 90 days, and then the blood and liver tissues were collected to determine biochemical parameter, gene expression and protein level. The results showed that HFD feeding signally increase...
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#1Rui Jia (Chinese Academy of Fishery Sciences)H-Index: 5
#2Liping Cao (Chinese Academy of Fishery Sciences)H-Index: 10
Last. Guojun Yin (Chinese Academy of Fishery Sciences)H-Index: 9
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Last. Paola Dongiovanni (Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico)H-Index: 39
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Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic liver disease and ranges from steatosis to steatohepatitis and to liver fibrosis. Lipotoxicity in hepatocytes, elevated oxidative stress and the activation of proinflammatory mediators of Kupffer cells, and fibrogenic pathways of activated hepatic stellate cells can contribute to the development of NAFLD. MicroRNAs (miRs) play a crucial role in the dysregulated metabolism and inflammatory signaling connected with NAFLD and it...
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BACKGROUND: microRNAs (miRNAs) post-transcriptionally regulate gene expression and act as important modulators of cholesterol homeostasis. OBJECTIVE: The study explores the mechanism by which miRNAs mediate high fat-induced changes of cholesterol metabolism in yellow catfish. METHODS: Yellow catfish (weight: 3.79 +/- 0.16 g, 3 mo old, mixed sex) were fed 2 diets containing lipids at 11.3% [control (CON)] or 15.4% [high-fat diet (HFD)] (by weight) for 8 wk. Cholesterol content was measured; hemat...
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