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Phenotyping for Nitrogen Use Efficiency: Rice Genotypes Differ in N-Responsive Germination, Oxygen Consumption, Seed Urease Activities, Root Growth, Crop Duration, and Yield at Low N

Published on Oct 1, 2018in Frontiers in Plant Science4.11
· DOI :10.3389/fpls.2018.01452
Narendra Sharma1
Estimated H-index: 1
(IP: Guru Gobind Singh Indraprastha University),
Vimlendu Bhushan Sinha4
Estimated H-index: 4
(IP: Guru Gobind Singh Indraprastha University)
+ 5 AuthorsNandula Raghuram13
Estimated H-index: 13
(IP: Guru Gobind Singh Indraprastha University)
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Abstract
The biological improvement of fertilizer nitrogen use efficiency (NUE) is hampered by the poor characterization of the phenotype and genotype for crop N response and NUE. In an attempt to identify phenotypic traits for N-response and NUE in the earliest stages of plant growth, we analysed the N-responsive germination, respiration, urease activities and root/shoot growth of 21 Indica genotypes of rice (O. sativa var. Indica). We found that N delays germination from 0 to 12 hrs in a genotype-dependent and source-dependent manner, especially with urea and nitrate. We identified contrasting groups of fast germinating genotypes such as Aditya, Nidhi and Swarnadhan, which were also least delayed by N and slow germinating genotypes such as Panvel1, Triguna and Vikramarya, which were also most delayed by N. Oxygen uptake measurements in the seeds of contrasting genotypes revealed that they were affected by N source in accordance with germination rates, especially with urea. Germinating seeds were found to have endogenous urease activity, indicating the need to explore genotypic differences in the effective urea uptake and metabolism, which remain unexplored so far. Urea was found to significantly inhibit early root growth in all genotypes but not shoot growth. Field evaluation of 15 of the above genotypes clearly showed that germination rates, crop duration and yield are linked to NUE. Slow germinating genotypes had longer crop duration and higher yield even at lower N, indicating their higher N use efficiency, relative to fast germinating or short duration genotypes. Moreover, longer duration genotypes suffered lesser yield losses at reduced N levels as compared to short duration genotypes, which is also a measure of their NUE. Together, these results indicate the potential of germination rates, crop duration, urea utilization and its effect on root growth in the development of novel phenotypic traits for screening genotypes and crop improvement for N use efficiency, at least in rice.
  • References (38)
  • Citations (1)
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References38
Newest
Published on Jan 1, 2018
Vikas Kumar Mandal1
Estimated H-index: 1
(IP: Guru Gobind Singh Indraprastha University),
Narendra Sharma1
Estimated H-index: 1
(IP: Guru Gobind Singh Indraprastha University),
Nandula Raghuram13
Estimated H-index: 13
(IP: Guru Gobind Singh Indraprastha University)
Unused fertilizers are the single largest source of nitrous oxide greenhouse gas emissions globally, apart from causing N-pollution in ground and surface waters and eutrophication. While short-term improvements in N-use efficiency (NUE) can be made by agronomic practices, long-term crop improvement is only possible through biological interventions. The lack of clearly defined phenotype and genotype has delayed this process till recently, but the advent of omics and reverse genetics is opening up...
Published on Dec 1, 2017in Scientific Reports4.01
Daxing Wen1
Estimated H-index: 1
(SDAU: Shandong Agricultural University),
Haicheng Xu1
Estimated H-index: 1
(SDAU: Shandong Agricultural University)
+ 3 AuthorsChunqing Zhang1
Estimated H-index: 1
(SDAU: Shandong Agricultural University)
Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was to study the mechanism of rapid seed germination and explore which pathways and genes play critical roles in radicle protrusion. Anatomical data revealed that seed protein content affected endosperm structure...
Published on May 1, 2017in Journal of Experimental Botany5.36
Lucas Gent1
Estimated H-index: 1
(Lancaster University),
Brian G. Forde47
Estimated H-index: 47
(Lancaster University)
The primary processes that contribute to the efficient capture of soil nitrate are the development of a root system that effectively explores the soil and the expression of high-affinity nitrate uptake systems in those roots. Both these processes are highly regulated to take into account the availability and distribution of external nitrate pools and the endogenous N status of the plant. While significant progress has been made in elucidating the early steps in sensing and responding to external...
Published on May 1, 2017in Annals of Botany3.45
Quan Xie5
Estimated H-index: 5
(NAU: Nanjing Agricultural University),
Kurukulasuriya M. C. Fernando1
Estimated H-index: 1
(University of Ruhuna)
+ 1 AuthorsDebbie L. Sparkes20
Estimated H-index: 20
(University of Nottingham)
Background and Aims: Plant roots growing underground are critical for soil resource acquisition, anchorage and plant-environment interactions. In wheat (Triticum aestivum), however, the target root traits to improve yield potential still remain largely unknown. This study aimed to identify traits of seedling root system architecture (RSA) associated with yield and yield components in 226 recombinant inbred lines (RILs) derived from a cross between the bread wheat Triticum aestivum ‘Forno’ (small...
Published on Dec 31, 2016in Seed Science and Technology0.51
A. Schnadelbach1
Estimated H-index: 1
,
L. Veiga-Barbosa1
Estimated H-index: 1
+ 2 AuthorsFélix Pérez-García16
Estimated H-index: 16
Senecio coincyi (Asteraceae) is a threatened endemic plant of central and north western Spain with a very narrow occurrence. In this study, germination tests under controlled conditions of light and temperature were carried out to evaluate interpopulation variation in the germination patterns of S. coincyi cypselas from 12 populations. The effects of nitrate (1, 5, 10, 25 and 50 mM KNO3) on percentage and rate of germination were also determined. Untreated cypselas of S. coincyi reached very hig...
Published on Dec 1, 2016in Environmental and Experimental Botany3.71
Xuejie Wan2
Estimated H-index: 2
(NWAFU: Northwest A&F University),
Wei Wu3
Estimated H-index: 3
(NWAFU: Northwest A&F University)
+ 3 AuthorsYuncheng Liao12
Estimated H-index: 12
(NWAFU: Northwest A&F University)
Abstract Poor seed germination and early seedling growth caused by ammonia toxicity following urea application are major constraints for wheat production. This study aims to determine ammonia volatilization caused by urea and its damage to seed germination, early seedling growth, and its associated physiological mechanism. Two petri dish experiments were conducted under four nitrogen (N) application rates (0, 0.58, 1.16 and 1.75 mg N g −1 soil) for six wheat varieties that differ in their ammoni...
Published on Jun 21, 2016in Proteome
Malgorzata Czarna4
Estimated H-index: 4
,
Marta Kolodziejczak2
Estimated H-index: 2
,
Hanna Janska17
Estimated H-index: 17
Seed germination is considered to be one of the most critical phases in the plant life cycle, establishing the next generation of a plant species. It is an energy-demanding process that requires functioning mitochondria. One of the earliest events of seed germination is progressive development of structurally simple and metabolically quiescent promitochondria into fully active and cristae-containing mitochondria, known as mitochondrial biogenesis. This is a complex and tightly regulated process,...
Published on Apr 1, 2016in Plant and Cell Physiology3.93
Takatoshi Kiba30
Estimated H-index: 30
,
Anne Krapp2
Estimated H-index: 2
(Université Paris-Saclay)
Nitrogen availability is a major factor determining plant growth and productivity. Plants acquire nitrogen nutrients from the soil through their roots mostly in the form of ammonium and nitrate. Since these nutrients are scarce in natural soils, plants have evolved adaptive responses to cope with the environment. One of the most important responses is the regulation of nitrogen acquisition efficiency. This review provides an update on the molecular determinants of two major drivers of the nitrog...
Published on Feb 1, 2016in Plant Physiology6.30
Bianka Steffens16
Estimated H-index: 16
,
Amanda Rasmussen11
Estimated H-index: 11
(University of Nottingham)
Adventitious roots are plant roots that form from any nonroot tissue and are produced both during normal development (crown roots on cereals and nodal roots on strawberry [Fragaria spp.]) and in response to stress conditions, such as flooding, nutrient deprivation, and wounding. They are important economically (for cuttings and food production), ecologically (environmental stress response), and for human existence (food production). To improve sustainable food production under environmentally ex...
Published on Jun 1, 2015in Journal of Experimental Botany5.36
Pengcheng Li3
Estimated H-index: 3
(CAU: China Agricultural University),
Fanjun Chen24
Estimated H-index: 24
(CAU: China Agricultural University)
+ 7 AuthorsLixing Yuan26
Estimated H-index: 26
(CAU: China Agricultural University)
That root system architecture (RSA) has an essential role in nitrogen acquisition is expected in maize, but the genetic relationship between RSA and nitrogen use efficiency (NUE) traits remains to be elucidated. Here, the genetic basis of RSA and NUE traits was investigated in maize using a recombination inbred line population that was derived from two lines contrasted for both traits. Under high-nitrogen and low-nitrogen conditions, 10 NUE- and 9 RSA-related traits were evaluated in four field ...
Cited By1
Newest
Published on Apr 9, 2019in Scientific Reports4.01
Navjyoti Chakraborty3
Estimated H-index: 3
(IP: Guru Gobind Singh Indraprastha University),
Kostya Kanyuka22
Estimated H-index: 22
(Rothamsted Research)
+ 6 AuthorsNandula Raghuram13
Estimated H-index: 13
(IP: Guru Gobind Singh Indraprastha University)
G-protein signaling components have been attributed many biological roles in plants, but the extent of involvement of G-protein coupled receptor 1 (GCR1) with the Gα (GPA1) remained unknown. To address this, we have performed transcriptomic analyses on Arabidopsis gpa1-5gcr1-5 double mutant and identified 656 differentially expressed genes (DEGs). MapMan and Gene Ontology analyses revealed global transcriptional changes associated with external stimulus, cell wall organization/biogenesis and sec...
Narendra Sharma1
Estimated H-index: 1
(IP: Guru Gobind Singh Indraprastha University),
Surekha Kuchi (ICAR: Indian Council of Agricultural Research)+ 1 AuthorsNandula Raghuram13
Estimated H-index: 13
(IP: Guru Gobind Singh Indraprastha University)
ABSTRACTResearch for nutrient optimization and crop nutrient use efficiency requires precise control on soil nutrient status. While nutrient-depleted soils are preferable to artificial soils or hyd...
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