Match!

Co-circulation of genetically diverse population of vaccine related and unrelated respiratory mycoplasmas and viruses in UK poultry flocks with health or production problems

Published on Nov 1, 2018in Veterinary Microbiology2.79
· DOI :10.1016/j.vetmic.2018.09.009
Christopher Ball2
Estimated H-index: 2
(University of Liverpool),
Anne Forrester6
Estimated H-index: 6
(University of Liverpool),
Kannan Ganapathy12
Estimated H-index: 12
(University of Liverpool)
Abstract
Abstract Respiratory diseases continue to have a major impact on poultry health, welfare and productivity. However, little information is available on their current status in UK poultry flocks. We investigated the presence of four economically important respiratory pathogens in healthy or problematic flocks; infectious bronchitis virus (IBV), avian metapneumovirus (aMPV), Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae (Ms). Samples from 131 UK poultry flocks were received during the 12 month study period. Oropharyngeal (OP) swabs were taken from eight birds per flock and accompanied with flock health information. The study included 118 chicken, 6 pheasant and 5 turkey flocks, and 1 quail and 1 partridge flock. Chicken flocks were of layers (n = 98), broilers (n = 15), breeders (n = 3) and undisclosed (n = 2). Flock ages ranged from 3 to 72 weeks old, and the average flock size was 17,633 birds. PCR detected 65 (49.6%), 59 (45%) and 8 (6.1%) flocks as positive for IBV, Mg/Ms and aMPV respectively. Analysis of the mgc2 gene of the Mg isolates revealed high similarities to Mg TS-11 and Mg 6/85. Further gene analysis found that the TS-11-like isolates were unrelated to the TS-11 vaccine. Multi-locus sequence typing (MLST) analysis identified the majority of positive Ms as ST21, along with ST2 (MS-H-like), ST6 and ST43. IBV S1 gene sequencing identified strains as 793B (66.7%), Arkansas (23.8%) and Massachusetts (9.5%). All aMPV positive samples belonged to subtype B. Findings indicate that over half of the flocks sampled were positive for at least one of the four vaccine or field strains of mycoplasmas or viruses.
  • References (31)
  • Citations (1)
References31
Newest
#1Camir Ricketts (UGA: University of Georgia)H-Index: 1
#2Larissa Pickler (UFPR: Federal University of Paraná)H-Index: 5
Last.Naola Ferguson-Noel (UGA: University of Georgia)H-Index: 8
view all 6 authors...
#1Mohamed El-Gazzar (OSU: Ohio State University)H-Index: 3
#2Mostafa Ghanem (OSU: Ohio State University)H-Index: 3
Last.Richard D. Slemons (OSU: Ohio State University)H-Index: 23
view all 6 authors...
#1Christopher Ball (University of Liverpool)H-Index: 2
#2Anne Forrester (University of Liverpool)H-Index: 6
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
view all 3 authors...
#1Kannan Ganapathy (University of Liverpool)H-Index: 12
#2Christopher Ball (University of Liverpool)H-Index: 2
Last.Anne Forrester (University of Liverpool)H-Index: 6
view all 3 authors...
#1Thunai Al-Shekaili (University of Liverpool)H-Index: 1
#2Matthew Baylis (University of Liverpool)H-Index: 39
Last.Kannan Ganapathy (University of Liverpool)H-Index: 12
view all 3 authors...
#1Ra Cha (USDA: United States Department of Agriculture)H-Index: 2
#2Qingzhong Yu (USDA: United States Department of Agriculture)H-Index: 13
Last.L. Zsak (USDA: United States Department of Agriculture)H-Index: 28
view all 3 authors...
View next paperPrevalence and molecular characterization of avian infectious bronchitis virus in poultry flocks in Morocco from 2010 to 2014 and first detection of Italy 02 in Africa