Crystal structure of yeast xylose reductase in complex with a novel NADP‐DTT adduct provides insights into substrate recognition and catalysis

Aldose reductases ( AR s) belonging to the aldo‐keto reductase ( AKR ) superfamily catalyze the conversion of carbonyl substrates into their respective alcohols. Here we report the crystal structures of the yeast Debaryomyces nepalensis xylose reductase ( Dn XR , AKR 2B10) in the apo form and as a ternary complex with a novel NADP ‐ DTT adduct. Xylose reductase, a key enzyme in the conversion of xylose to xylitol, has several industrial...