Noise-free microbial colony counting method based on hyperspectral features of agar plates

A noise-free bacterial colony counting method identifying noise (i.e., sausage, bacon, and millet fragments) with similar colors or shapes to those of colonies was developed for food quality assessment. First, spectral features corresponding to colony cluster regions and background regions (agar medium and food fragments) were extracted after collection of hyperspectral images. A cluster-segmenting calibration model that could identify colony...