P139 Flow crossmatch thresholds: Where (and how) do we draw the line?

Flow cytometry crossmatch (FCXM) is used to assess pre-transplant immunologic risk. Each laboratory must establish FCXM positive thresholds but the method to do so is not standardized. Many factors affect FCXM and its interpretation. Our aim was to compare methods of calculating FCXM thresholds and evaluate the impact of cell source and pronase. Threshold study (TS) data were collected by FCXM with healthy control sera negative for HLA...