Gelatin as a convenient surrogate protein to model the in vitro effects of advanced glycation end-product formation

Protein glycation has been implicated in skin ageing and several other disease states; however, the slow rate of glycation end‐product formation makes in vitro studies challenging and often impractical. Gelatin, a denatured form of collagen, was identified as a convenient glycation surrogate amenable to cell culture conditions. The suitability of glycated gelatin to model the effects of AGE formation was verified using RAW 264.7 macrophages...