Application of CRISPR/Cas9 toTragopogon(Asteraceae), an evolutionary model for the study of polyploidy

Tragopogon (Asteraceae) is an excellent natural system for studies of recent polyploidy. Development of an efficient CRISPR/Cas9‐based genome editing platform in Tragopogon will facilitate novel studies of the genetic consequences of polyploidy. Here, we report our initial results of developing CRISPR/Cas9 in Tragopogon . We have established a feasible tissue culture and transformation protocol for Tragopogon . Through protoplast transient...