Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency

Although endonuclease-mediated genome editing techniques offer significant improvement over traditional methods, they are still ineffective for introduction of large DNA sequences. Recently in Nature Biotechnology, Gu et al., 2018Gu B. Posfai E. Rossant J. Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.Nat. Biotechnol. 2018; 36: 632-637Crossref PubMed Scopus (143) Google Scholar developed a...