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Reversible permeabilization of the mitochondrial membrane promotes human cardiomyocyte differentiation from embryonic stem cells

Published on Jan 1, 2019in Journal of Cellular Physiology4.522
路 DOI :10.1002/jcp.26758
Parisa Ghiasi3
Estimated H-index: 3
(TMU: Tarbiat Modares University),
Saman Hosseinkhani28
Estimated H-index: 28
(TMU: Tarbiat Modares University)
+ 4 AuthorsHossein Baharvand44
Estimated H-index: 44
(University of Science and Culture)
Abstract
  • References (51)
  • Citations (2)
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References51
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#1Tao YunH-Index: 1
#2Kaiwen YuH-Index: 9
Last. Xuejun Jiang (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 33
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Abstract The p53 tumor suppressor controls cell growth, metabolism, and death by regulating the transcription of various target genes. The target-specific transcriptional activity of p53 is highly regulated. Here we demonstrate that acetylation of p53 at Lys-120 up-regulates its transcriptional activity toward Apaf-1, a core component in the mitochondrial apoptotic pathway, and thus sensitizes caspase activation and apoptosis. We found that histone deacetylase (HDAC) inhibitors, including butyra...
13 CitationsSource
#1Hananeh Fonoudi (VCCRI: Victor Chang Cardiac Research Institute)H-Index: 4
#2Hassan Ansari (Academic Center for Education, Culture and Research)H-Index: 5
Last. Hossein Baharvand (Academic Center for Education, Culture and Research)H-Index: 44
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Recent advances in the generation of cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs), in conjunction with the promising outcomes from preclinical and clinical studies, have raised new hopes for cardiac cell therapy. We report the development of a scalable, robust, and integrated differentiation platform for large-scale production of hPSC-CM aggregates in a stirred suspension bioreactor as a single-unit operation. Precise modulation of the differentiation process by small molecule ...
49 CitationsSource
#1Shiva Akbari-Birgani (TMU: Tarbiat Modares University)H-Index: 3
#2Saman Hosseinkhani (TMU: Tarbiat Modares University)H-Index: 28
Last. Hossein Baharvand (University of Science and Culture)H-Index: 44
view all 4 authors...
Differentiation is an inseparable process of development in multicellular organisms. Mouse embryonic stem cells (mESCs) represent a valuable research tool to conduct in vitro studies of cell differentiation. Apoptosis as a well known cell death mechanism shows some common features with cell differentiation, which has caused a number of ambiguities in the field. The research question here is how cells could differentiate these two processes from each other. We have investigated the role of the mi...
24 CitationsSource
#1Daohong Zhou (University of Arkansas for Medical Sciences)H-Index: 37
#2Lijian Shao (University of Arkansas for Medical Sciences)H-Index: 17
Last. Douglas R. Spitz (Roy J. and Lucille A. Carver College of Medicine)H-Index: 66
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Reactive oxygen species (ROS) play an important role in determining the fate of normal stem cells. Low levels of ROS are required for stem cells to maintain quiescence and self-renewal. Increases in ROS production cause stem cell proliferation/differentiation, senescence, and apoptosis in a dose-dependent manner, leading to their exhaustion. Therefore, the production of ROS in stem cells is tightly regulated to ensure that they have the ability to maintain tissue homeostasis and repair damaged t...
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#1Ng Shyh-Chang (Harvard University)H-Index: 22
#2George Q. Daley (Harvard University)H-Index: 108
Last. Lewis C. Cantley (Cornell University)H-Index: 172
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Recent advances in metabolomics and computational analysis have deepened our appreciation for the role of specific metabolic pathways in dictating cell fate. Once thought to be a mere consequence of the state of a cell, metabolism is now known to play a pivotal role in dictating whether a cell proliferates, differentiates or remains quiescent. Here, we review recent studies of metabolism in stem cells that have revealed a shift in the balance between glycolysis, mitochondrial oxidative phosphory...
278 CitationsSource
#1Victoria A. Rafalski (Stanford University)H-Index: 11
#2Elena Mancini (Stanford University)H-Index: 12
Last. Anne Brunet (Stanford University)H-Index: 64
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Metabolism is influenced by age, food intake, and conditions such as diabetes and obesity. How do physiological or pathological metabolic changes influence stem cells, which are crucial for tissue homeostasis? This Commentary reviews recent evidence that stem cells have different metabolic demands than differentiated cells, and that the molecular mechanisms that control stem cell self-renewal and differentiation are functionally connected to the metabolic state of the cell and the surrounding st...
83 CitationsSource
Current protocols for the scalable suspension culture of human pluripotent stem cells (hPSCs) are limited by multiple biological and technical challenges that need to be addressed before their use in clinical trials. To overcome these challenges, we have developed a novel bioprocess platform for large-scale expansion of human embryonic and induced pluripotent stem cell lines as three-dimensional size-controlled aggregates. This novel bioprocess utilizes the stepwise optimization of both static a...
86 CitationsSource
#1Raluca Dumitru (UNC: University of North Carolina at Chapel Hill)H-Index: 9
#2Vivian Gama (UNC: University of North Carolina at Chapel Hill)H-Index: 15
Last. Mohanish Deshmukh (UNC: University of North Carolina at Chapel Hill)H-Index: 38
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Summary Human embryonic stem (hES) cells activate a rapid apoptotic response after DNA damage but the underlying mechanisms are unknown. A critical mediator of apoptosis is Bax, which is reported to become active and translocate to the mitochondria only after apoptotic stimuli. Here we show that undifferentiated hES cells constitutively maintain Bax in its active conformation. Surprisingly, active Bax was maintained at the Golgi rather than at the mitochondria, thus allowing hES cells to effecti...
86 CitationsSource
#1C茅cile Martel (University of Paris-Sud)H-Index: 18
#2Le Ha Huynh (University of Paris-Sud)H-Index: 2
Last. Catherine Brenner (University of Paris-Sud)H-Index: 55
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Mitochondria are fascinating organelles, which fulfill multiple cellular functions, as diverse as energy production, fatty acid 尾 oxidation, reactive oxygen species (ROS) production and detoxification, and cell death regulation. The coordination of these functions relies on autonomous mitochondrial processes as well as on sustained cross-talk with other organelles and/or the cytosol. Therefore, this implies a tight regulation of mitochondrial functions to ensure cell homeostasis. In many disease...
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#1Parisa Ghiasi (TMU: Tarbiat Modares University)H-Index: 3
#2Saman Hosseinkhani (TMU: Tarbiat Modares University)H-Index: 28
Last. Khosro Khajeh (TMU: Tarbiat Modares University)H-Index: 26
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AbstractObjectives: Several lines of evidence suggest that mitochondrial dysfunction is involved in amyotrophic lateral sclerosis (ALS), but despite the fact that mitochondria play a central role in excitotoxicity, oxidative stress, and apoptosis, the intimate underlying mechanism linking mitochondrial defects to motor neuron degeneration in ALS still remains elusive. This study was performed to assess the mitochondrial respiratory chain dysfunction and cellular energy index (ATP/ADP ratio) in l...
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#1Sadat Dokanehiifard (TMU: Tarbiat Modares University)H-Index: 4
#2Bahram Mohammad Soltani (TMU: Tarbiat Modares University)H-Index: 10
Last. Saman Hosseinkhani (TMU: Tarbiat Modares University)H-Index: 28
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Abstract Dispose of unnecessary cells in multicellular organism take place through apoptosis as a mode of programmed cell death (PCD). This process is triggered through two main pathway including extrinsic pathway or death receptor pathway and intrinsic or mitochondrial pathway. An alternative role for mitochondrial pathway of cell death is its involvement in cell differentiation. Biochemistry of cell differentiation indicates a common origin for differentiation and apoptosis. miRNAs are a group...
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#1Yasaman-Sadat Borghei (TMU: Tarbiat Modares University)
#2Saman Hosseinkhani (TMU: Tarbiat Modares University)H-Index: 28
Abstract Abnormalities in apoptosis (More or less than normal rate) is a central factor to detect many human disorders such as many types of cancer and to show therapeutic potential of drugs. During apoptosis, oxidation of reduced gluthation (GSH) to its oxidized form, GSSG, results in a decreased GSH to GSSG ratio. Here, we used altered GSH/GSSG redox state and the release of Cyt c from mitochondria to cytosol as an indicator for apoptosis assay. This study reports a visual method for cell apop...
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#1Zahra Madadi (IASBS: Institute for Advanced Studies in Basic Sciences)H-Index: 1
#2Shiva Akbari-Birgani (IASBS: Institute for Advanced Studies in Basic Sciences)H-Index: 3
Last. Saeed Mohammadi (Tehran University of Medical Sciences)H-Index: 6
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Abstract Caspase family contains cysteine proteases involving in the key cellular processes, such as apoptosis, inflammation, and autophagy. There is a growing body of evidence that caspase family also plays a role in cellular differentiation. Evidence suggests that caspase-9 is among the most important members with non-apoptotic roles in the execution of differentiation. Since drug-induced differentiation in some types of cancer cells is a promising treatment, we have investigated caspase-9 act...
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