Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation
Abstract
It is known that the crystallizability of protein molecules may be improved by replacing their surface lysine residues with other residue types. Here an experimental method to identify surface lysine residues by NHS-biotin chemical modification combined with MALDI-TOF MS was proposed and was evaluated using PH1033 protein from Pyrococcus horikoshii. Interestingly, the biotinylation experiment with a protein-reagent molar ratio of 1:1 revealed...
Paper Details
Title
Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation
Published Date
Sep 1, 2018
Journal
Volume
557
Pages
46 - 58
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