B Cell Immune Repertoire Sequencing Identifies Pre-transplant Rejection Risk

Background The diversity of the B cell immune repertoire may drive humoral injury and rejection in organ transplantation (tx). Methods We utilized advances in next-generation sequencing of B cells and custom computational pipelines to study functionally correlated variations in the VDJ and CDR3 regions, examining 124 DNA and RNA samples from the same tx patient over serial times before tx, and at 6 and 24 mo post-tx. Each blood sample was paired...