Plasma Donor-Derived Cell-Free DNA Quantification by massively multiplex PCR Distinguishes Kidney Transplant Acute Rejection

Introduction Plasma donor-derived cell-free DNA (dd-cfDNA) has been implicated as a noninvasive marker for transplant (tx) rejection. dd-cfDNA evaluation requires differentiation of donor/recipient DNA by sequencing; recent studies have amplified hundreds of target SNPs to detect active rejection in kidney allografts with 59.3% sensitivity and 84.7% specificity. We measure thousands of informative SNPs to assess dd-cfDNA with high accuracy in a...