Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes

Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes. To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE). Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied. A Box-Behnken design (BBD) was applied to...