p53 Throws CRISPR a Curve

The efficacy of the powerful CRISPR-Cas9 genome-editing platform depends on DNA repair activities in the cells being targeted. Two new papers show that the low efficiency of targeting in some primary human cell lines is the result of p53-dependent cell arrest in response to the Cas9-induced break. This limitation must be overcome for some anticipated therapies. The efficacy of the powerful CRISPR-Cas9 genome-editing platform depends on DNA...