Study on the influence of lysozyme crystallization conditions on crystal properties in crystallizers of varied sizes when temperature is the manipulated variable

In this work, crystallization experiments were conducted in three different sizes of crystallizers (5 and 100 ml, and 1 L) to study the influence of temperature on the crystallization of lysozyme. Lysozyme solutions with concentrations of 40 and 30 g L−1 and 10% (w/w) NaCl were used. The temperature was reduced from 20 to 0 °C with various cooling rate and stirring speed. The data indicated that crystallization with cooling but without agitation...