Identifying metabolic elements that contribute to productivity of 1-propanol bioproduction using metabolomic analysis

Previously constructed Escherichia coli strains that produce 1-propanol use the native threonine pathway, or a heterologous citramalate pathway. However, based on the energy and cofactor requirements of each pathway, a combination of the two pathways produces synergistic effects that increase the theoretical maximum yield with a simultaneous unexplained increase in productivity. Identification of key factors that contribute to synergistic effect...