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Training Performed Above Lactate Threshold Decreases p53 and Shelterin Expression in Mice

Published on Sep 1, 2018in International Journal of Sports Medicine2.13
· DOI :10.1055/a-0631-3441
Verusca Najara de Carvalho Cunha6
Estimated H-index: 6
(UCB: Universidade Católica de Brasília),
Thiago Santos Rosa7
Estimated H-index: 7
(UCB: Universidade Católica de Brasília)
+ 5 AuthorsRosangela Vieira de Andrade7
Estimated H-index: 7
(UCB: Universidade Católica de Brasília)
Abstract
Telomere shortening is associated to sarcopenia leading to functional impairment during aging. There are mechanisms associated with telomere attrition, as well to its protection and repair. Physical training is a factor that attenuates telomere shortening, but little is known about the effects of different exercise intensities on telomere biology. Thus, we evaluated the effects of exercise intensity (moderate vs. high-intensity domain) on gene expression of senescence markers Checkpoint kinase 2 and tumor suppressor (Chk2 and p53, respectively), shelterin telomere repeat binding 1 and 2 (Trf1/Trf2), DNA repair (Xrcc5), telomerase reverse transcriptase (mTERT) and telomere length in middle aged mice. Three groups were studied: a control group (CTL) and two groups submitted to swimming at intensities below the lactate threshold (LI group) and above the lactate threshold (HI group) for 40 and 20 min respectively, for 12 weeks. After training, the HI group showed reduction in p53 expression in the muscle, and decreased shelterin complex expression when compared to LI group. No differences were observed between groups for mTERT expression and telomere length. Thus, exercise training in high-intensity domain was more effective on reducing markers of senescence and apoptosis. The higher intensity exercise training also diminished shelterin expression, with no differences in telomere length and mTERT expression. Such results possibly indicate a more effective DNA protection for the higher-intensity exercise training.
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References48
Newest
#1D. J. Harriss (LJMU: Liverpool John Moores University)H-Index: 8
#2Alasdair MacSween (Teesside University)H-Index: 7
Last.Gregory Atkinson (Teesside University)H-Index: 10
view all 3 authors...
#1Andrew T. Ludlow (UMD: University of Maryland, College Park)H-Index: 16
#2Laila Gratidão (UCB: Universidade Católica de Brasília)H-Index: 1
Last.Stephen M. Roth (UMD: University of Maryland, College Park)H-Index: 39
view all 5 authors...
#1Bertrand Fougère (University of Toulouse)H-Index: 12
#2Eric Boulanger (Lille University of Science and Technology)H-Index: 17
Last.Matteo Cesari (University of Toulouse)H-Index: 63
view all 5 authors...
#1Harold H. Lee (Brown University)H-Index: 3
#2Jessica A. Emerson (Brown University)H-Index: 4
Last.David Williams (Brown University)H-Index: 59
view all 3 authors...
#1Cesare Granata (VU: Victoria University, Australia)H-Index: 10
#2Rodrigo S F Oliveira (VU: Victoria University, Australia)H-Index: 6
Last.David Bishop (VU: Victoria University, Australia)H-Index: 58
view all 5 authors...
#1Joshua Denham (UNE: University of New England (Australia))H-Index: 10
#2Brendan J. O’Brien (FedUni: Federation University Australia)H-Index: 17
Last.Fadi J. Charchar (FedUni: Federation University Australia)H-Index: 29
view all 5 authors...
#1Adeel Safdar (McMaster University)H-Index: 27
#2Konstantin Khrapko (NU: Northeastern University)H-Index: 4
Last.Mark A. Tarnopolsky (McMaster University)H-Index: 96
view all 19 authors...
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