Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing have led to the use of long single-stranded DNA (lssDNA) molecules for generating conditional mutations. However, there is still limited available data on the efficiency and reliability of this method. We generated conditional mouse alleles using lssDNA donor templates and performed extensive characterization of...