From microscale phase screening to bulk evaporative crystallization of proteins

Protein phase diagrams obtained from material-efficient microscale phase screenings can be used successfully for designing a macroscale preparative protein crystallization process to gain the crystal morphology desired. In this experimental study, the phase behavior of three globular proteins: hen egg white lysozyme, bovine serum albumin, and alcohol dehydrogenase from Lactobacillus brevis, is evaluated by isothermal microbatch phase screenings...