Improved ssDNA recombineering for rapid and efficient pathway engineering in Corynebacterium glutamicum
Volume: 93, Issue: 12, Pages: 3535 - 3542
Published: Jul 4, 2018
Abstract
BACKGROUND Corynebacterium glutamicum is an important microorganism for the industrial production of high value‐added compounds. Therefore, powerful genetic engineering tools are required for metabolic engineering of C. glutamicum . RESULTS In this study, the application of ssDNA recombineering was extended to realize efficient and rapid genetic engineering of C. glutamicum . The efficiency of one‐step ssDNA recombineering reached 60% without...
Paper Details
Title
Improved ssDNA recombineering for rapid and efficient pathway engineering in Corynebacterium glutamicum
Published Date
Jul 4, 2018
Volume
93
Issue
12
Pages
3535 - 3542
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