Optimization of phenol-chloroform RNA extraction

Volume: 5, Pages: 599 - 608
Published: Jan 1, 2018
Abstract
Accurate and reliable analysis of gene expression depends on the extraction of pure and high-quality RNA. However, while the conventional phenol-chloroform RNA extraction is preferable over silica-based columns, particularly when cost is a concern or higher RNA yield is desired, it can result in significant RNA contamination. Contaminants including excess phenol, chloroform, or salts, can have significant impacts on downstream applications,...
Paper Details
Title
Optimization of phenol-chloroform RNA extraction
Published Date
Jan 1, 2018
Journal
Volume
5
Pages
599 - 608
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