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Biomineralization of osteoblasts on DLC coated surfaces for bone implants

Published on Aug 1, 2018in Biointerphases2.009
· DOI :10.1116/1.5007805
Balasubramanian Subramanian21
Estimated H-index: 21
(CSIR: Council of Scientific and Industrial Research),
Thanka Rajan S1
Estimated H-index: 1
(CSIR: Council of Scientific and Industrial Research)
+ 4 AuthorsAvi Bendavid25
Estimated H-index: 25
(CSIRO: Commonwealth Scientific and Industrial Research Organisation)
Abstract
Diamond like carbon (DLC) films were deposited onto Ti6Al4V and Si wafer substrates by RF plasma enhanced chemical vapor deposition. The influence of dopants such as fluorine (F), silicon (Si), and nitrogen (N) on composition, structure, and biocompatibility was investigated. Ion scattering spectroscopy analysis revealed the presence of dopant atoms in the outer-most layers of the films. Raman studies showed that the position of the G-band shifts to higher frequencies with the fluorine and nitrogen content in the DLC film, whereas the incorporation of Si into DLC induces a decrease of the position of the G peak. The corrosion behavior was studied in simulated body fluid. A higher charge transfer resistance (Rct) was observed for the doped DLC films. The indirect cytotoxicity was performed using L929 fibroblast cells. The coated surfaces were hemocompatible when tested with red blood cells. DLC films were noncytotoxic to L929 cells over a 24 h exposure. Saos-2 osteoblast cell response to the doped and undoped DLC coated surfaces was studied in adhesion, proliferation, differentiation, and mineralization assays. The production of calcium and phosphate by cells on doped DLC, particularly, nitrogen doped DLC, was higher than that on undoped DLC.Diamond like carbon (DLC) films were deposited onto Ti6Al4V and Si wafer substrates by RF plasma enhanced chemical vapor deposition. The influence of dopants such as fluorine (F), silicon (Si), and nitrogen (N) on composition, structure, and biocompatibility was investigated. Ion scattering spectroscopy analysis revealed the presence of dopant atoms in the outer-most layers of the films. Raman studies showed that the position of the G-band shifts to higher frequencies with the fluorine and nitrogen content in the DLC film, whereas the incorporation of Si into DLC induces a decrease of the position of the G peak. The corrosion behavior was studied in simulated body fluid. A higher charge transfer resistance (Rct) was observed for the doped DLC films. The indirect cytotoxicity was performed using L929 fibroblast cells. The coated surfaces were hemocompatible when tested with red blood cells. DLC films were noncytotoxic to L929 cells over a 24 h exposure. Saos-2 osteoblast cell response to the doped and undo...
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#1Kaixiong GaoH-Index: 7
#2Xiaoli WeiH-Index: 1
Last. Junyan Zhang (CAS: Chinese Academy of Sciences)H-Index: 31
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Abstract Diamond like carbon (DLC) films are considered as exciting candidates for biomaterials owing to high hardness and chemical inertness. However, the high resistivity of pure DLC films limited further applications in bioelectronic field. The palladium and phosphorus doped DLC film was fabricated by electrochemical deposition from the ethanol solution of Tetrakis (triphenylphosphine) platinum. The structure of the film was composed of C P, C P, C H, sp2–C, sp3–C bonds and Pd nanoparticles. ...
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Abstract This work aims to investigate the chemical and biological responses of a-C:H films produced by PECVD technique onto substrate of Ti6Al4V alloy. The films produced were characterized morphologically and structurally by optical microscope (OM), SEM, AFM, EDX, FTIR Raman Spectroscopy (RS) and XPS. Afterwards, biocompatibility and cytotoxicity tests on osteoblastic cells and/or Human Peripheral Blood Mononuclear Cells (PBMCs) were performed on the Ti6Al4V alloy with coated and uncoated a-C:...
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#1Ivana KopovaH-Index: 6
#2Jakub Kronek (CTU: Czech Technical University in Prague)H-Index: 3
Last. Jaroslav FenclH-Index: 1
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Abstract Diamond-like carbon (DLC) coatings exhibit excellent mechanical properties and improve the smoothness and the wear characteristic of the metallic component of total joint replacements. Although DLC is considered to be highly biocompatible, the effect of implant wear debris must be carefully analyzed. Simulated loading can help to mimic the wear of the implant in the human body over time. The purpose of the study presented here is to observe the wear of trapeziometacarpal (TMC) total joi...
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