Live-Cell Imaging of Early Steps of Single HIV-1 Infection

Published on May 19, 2018in Viruses3.811
· DOI :10.3390/v10050275
Ashwanth C. Francis5
Estimated H-index: 5
Gregory B. Melikyan23
Estimated H-index: 23
Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection.
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