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Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics

Published on Apr 26, 2018in Molecules3.06
· DOI :10.3390/molecules23051013
Ping Zhang2
Estimated H-index: 2
,
Saina Li2
Estimated H-index: 2
+ 5 AuthorsBin Liu1
Estimated H-index: 1
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Abstract
Since the cost of Ophiocordyceps sinensis, an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products.
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  • References (51)
  • Citations (2)
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References51
Newest
Published on May 1, 2018in Journal of Pharmaceutical and Biomedical Analysis2.98
Lu Zhang2
Estimated H-index: 2
(Tianjin University of Traditional Chinese Medicine),
Yuanyuan Ge1
Estimated H-index: 1
(Tianjin University of Traditional Chinese Medicine)
+ 5 AuthorsYan-xu Chang13
Estimated H-index: 13
(Tianjin University of Traditional Chinese Medicine)
Abstract Columbianetin-β- d -glucopyranoside (CBG) and its metabolite columbianetin (CBN) are the bioactive constituents of Angelicae pubescentis radix (APR). They exhibit the anti-platelet aggregation, anti-inflammatory and analgesic properties. The absorption, distribution, metabolism and excretion (ADME) of CBG has not been reported to date. Both high-performance liquid chromatography with fluorescence detection and ultra high-performance liquid chromatography coupled with quadrupole-time-of-...
Published on Dec 1, 2017in Scientific Reports4.01
Michael Woolman4
Estimated H-index: 4
(U of T: University of Toronto),
Adam Gribble6
Estimated H-index: 6
(U of T: University of Toronto)
+ 8 AuthorsAlex Vitkin14
Estimated H-index: 14
(U of T: University of Toronto)
Spatially Targeted Mass Spectrometry (MS) analysis using survey scans with an imaging modality often requires consecutive tissue slices, because of the tissue damage during survey scan or due to incompatible sample preparation requirements between the survey modality and MS. We report two spatially targeted MS analysis workflows based on polarized light imaging guidance that use the same tissue sample for survey and targeted analysis. The first workflow is applicable for thin-slice analysis, and...
Published on Sep 18, 2017in Molecules3.06
Lian-Xian Guo4
Estimated H-index: 4
,
Xiao-Ming Xu6
Estimated H-index: 6
+ 2 AuthorsJiang-Hai Wang7
Estimated H-index: 7
Ophiocordyceps sinensis is one rare medicinal fungus produced in the Qinghai-Tibetan Plateau. Its quality and price varies hugely with different habitat, and its numerous substitutes have sprung up in functional food markets. This paper aims to discriminate the geographic origin of wild O. sinensis and its substitutes via element analyzer–isotope ratio mass spectrometry and gas chromatography–isotope ratio mass spectrometry. The δ13C values of major fatty acids in the lipids of O. sinensis are c...
Published on Jun 1, 2017in Journal of Visualized Experiments1.11
Salem1
Estimated H-index: 1
(MPG: Max Planck Society),
M. Bernach1
Estimated H-index: 1
(MPG: Max Planck Society)
+ 1 AuthorsPatrick Giavalisco37
Estimated H-index: 37
(MPG: Max Planck Society)
Understanding of complex biological systems requires the measurement, analysis and integration of multiple compound classes of the living cell, usually determined by transcriptomic, proteomic, metabolomics and lipidomic measurements. In this protocol, we introduce a simple method for the reproducible extraction of metabolites, lipids and proteins from biological tissues using a single aliquot per sample. The extraction method is based on a methyl tert-butyl ether: methanol: water system for liqu...
Published on Mar 1, 2017in Electrophoresis2.75
Niu J. Tan1
Estimated H-index: 1
(UPM: Universiti Putra Malaysia),
Leona Daniela Jeffery Daim2
Estimated H-index: 2
(UPM: Universiti Putra Malaysia)
+ 2 AuthorsKaruppiah Thilakavathy3
Estimated H-index: 3
(UPM: Universiti Putra Malaysia)
Effective protein extraction is essential especially in producing a well-resolved proteome on 2D-gels. A well-resolved placental cotyledon proteome, with good reproducibility, have allowed researchers to study the proteins underlying the physiology and pathophysiology of pregnancy. The aim of this study is to determine the best protein extraction protocol for the extraction of protein from placental cotyledons tissues for a two-dimensional gel electrophoresis (2D-GE). Based on widely used protei...
Published on Mar 1, 2017in Electrophoresis2.75
Kevin M. Legg2
Estimated H-index: 2
(DU: University of Denver),
Roger Powell14
Estimated H-index: 14
(CU: University of Colorado Boulder)
+ 2 AuthorsPhillip B. Danielson18
Estimated H-index: 18
(DU: University of Denver)
Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA profile has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analyses. Previous proteome studies compiled panels of “candidate biomarkers” specific to each of five body fluids (i.e., peripheral blood, vaginal/menstrual fluid, seminal fluid, urine, and saliva...
Changwen Du15
Estimated H-index: 15
(CAS: Chinese Academy of Sciences),
Jianmin Zhou17
Estimated H-index: 17
(CAS: Chinese Academy of Sciences),
Jianfeng Liu1
Estimated H-index: 1
Abstract With increased demand for Cordyceps sinensis it needs rapid methods to meet the challenge of identification raised in quality control. In this study Cordyceps sinensis from four typical natural habitats in China was characterized by depth-profiling Fourier transform infrared photoacoustic spectroscopy. Results demonstrated that Cordyceps sinensis samples resulted in typical photoacoustic spectral appearance, but heterogeneity was sensed in the whole sample; due to the heterogeneity Cord...
Published on Jan 3, 2017in PLOS ONE2.78
Yun-Fei Komi1
Estimated H-index: 1
,
Jian-Ching Liau3
Estimated H-index: 3
+ 8 AuthorsHsin-Chih Lai30
Estimated H-index: 30
The caterpillar fungus Ophiocordyceps sinensis (previously called Cordyceps sinensis) has been used for centuries in Asia as a tonic to improve health and longevity. Recent studies show that O. sinensis produces a wide range of biological effects on cells, laboratory animals and humans, including anti-fatigue, anti-infection, anti-inflammatory, antioxidant, and anti-tumor activities. In view of the rarity of O. sinensis fruiting bodies in nature, cultivation of its anamorph mycelium represents a...
Published on Jan 1, 2017in Pharmacognosy Research
Chun-Hong Li4
Estimated H-index: 4
(Chongqing University),
Hua-Li Zuo4
Estimated H-index: 4
(UM: University of Macau)
+ 6 AuthorsFeng-Qing Yang24
Estimated H-index: 24
(Chongqing University)
Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS. Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas. Materials and Methods: Proteins from 26 different producing areas were extracted by Tris...
Cited By2
Newest
Published on Jul 1, 2019in Journal of Pharmaceutical and Biomedical Analysis2.98
Wei Jia6
Estimated H-index: 6
(Shaanxi University of Science and Technology),
Lin Shi1
Estimated H-index: 1
(Shaanxi University of Science and Technology)
+ 2 AuthorsXiaogang Chu10
Estimated H-index: 10
(Shaanxi University of Science and Technology)
Abstract Cordyceps sinensis is a traditional Chinese food. A high-throughput screening and quantitative method based on hydrophilic interaction chromatography coupled to quadrupole/Orbitrap high resolution mass spectrometry has been proposed for the joint measurement of nucleosides and their corresponding nucleotides in thirty-two Cordyceps sinensis samples. A total of thirty-two natural samples from six regions were enrolled. A multiple-step analysis strategy, including features extraction, noi...
Yao Chang-liang , Qian Zheng-Ming < + 4 AuthorsGuo Dean30
Estimated H-index: 30
Characterization of aqueous extract in traditional Chinese medicine (TCM) is challenging due to the poor retention of the analytes on conventional C18 columns. This study presents a systematic characterization method based on a rapid chromatographic separation (8 min) on a polar-modified C18 (Waters Cortecs T3) column of aqueous extract of Cordyceps sinensis. UHPLC-HRMS method was used to profile components in both untargeted and targeted manners by full MS/PIL/dd-MS2 acquisition approach. The c...
Published on Mar 21, 2019in Molecules3.06
The term “Proteomics” refers to the characterization of the proteome, that is, all proteins present in a biological system [...]
Published on Oct 29, 2018in Molecules3.06
Lian-Xian Guo4
Estimated H-index: 4
,
Gui-Wei Zhang1
Estimated H-index: 1
+ 2 AuthorsJiang-Hai Wang7
Estimated H-index: 7
Ophiocordyceps sinensis has been utilized in China and adjacent countries for thousands of years as a rare functional food to promote health and treat diverse chronic diseases. In recent years, adulterants are usually identified in the processed products of wild O. sinensis. However, the effective adulteration examination has to be additionally performed except their routine test, and accordingly is time- and money-consuming. Recently, arsenic determination has become a necessary test for confir...
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