Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics.

Published on Apr 26, 2018in Molecules3.06
· DOI :10.3390/MOLECULES23051013
Ping Zhang1
Estimated H-index: 1
(Beijing University of Chinese Medicine),
Ping Zhang2
Estimated H-index: 2
(Beijing University of Chinese Medicine)
+ 5 AuthorsBin Liu1
Estimated H-index: 1
Since the cost of Ophiocordyceps sinensis, an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products.
Figures & Tables
  • References (65)
  • Citations (6)
📖 Papers frequently viewed together
1 Author (Zheng Yuguo)
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Lu Zhang (Tianjin University of Traditional Chinese Medicine)H-Index: 2
#2Yuanyuan Ge (Tianjin University of Traditional Chinese Medicine)H-Index: 2
Last. Yan-xu Chang (Tianjin University of Traditional Chinese Medicine)H-Index: 16
view all 8 authors...
Abstract Columbianetin-β- d -glucopyranoside (CBG) and its metabolite columbianetin (CBN) are the bioactive constituents of Angelicae pubescentis radix (APR). They exhibit the anti-platelet aggregation, anti-inflammatory and analgesic properties. The absorption, distribution, metabolism and excretion (ADME) of CBG has not been reported to date. Both high-performance liquid chromatography with fluorescence detection and ultra high-performance liquid chromatography coupled with quadrupole-time-of-...
4 CitationsSource
#1Lili Guo (UPenn: University of Pennsylvania)H-Index: 15
#2Qingqing Wang (UPenn: University of Pennsylvania)H-Index: 9
Last. Ian A. Blair (UPenn: University of Pennsylvania)H-Index: 61
view all 10 authors...
Friedreich’s ataxia (FA) is an autosomal recessive disease caused by an intronic GAA triplet expansion in the FXN gene, leading to reduced expression of the mitochondrial protein frataxin. FA is estimated to affect 1 in 50 000 with a mean age of death in the fourth decade of life. There are no approved treatments for FA, although experimental approaches, which involve up-regulation or replacement of frataxin protein, are being tested. Frataxin is undetectable in serum or plasma, and whole blood ...
8 CitationsSource
#1Ke-Chun Bai (NTU: National Taiwan University)H-Index: 1
#2Fuu Sheu (NTU: National Taiwan University)H-Index: 23
Abstract Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase ...
11 CitationsSource
#1Ke ZanH-Index: 2
#2Li-Li HuangH-Index: 1
Last. Wen-Jia LiH-Index: 1
view all 8 authors...
: This study is to establish the HPLC specific chromatogram and determine four main nucleosides of wild and cultivated Cordyceps sinensis. Uridine, inosine, guanosine and adenosine were selected as reference substance. HPLC analysis was performed on a Waters XSelect HSS T3 C₁₈ (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water(A)-acetonitrile (B) at a flow rate of 0.6 mL•min⁻¹ (0-5 min,0% B;5-15 min,0%-10% B, 15-30 min,10%-20% B, 30-33 min, 20%-50% B, 33-35 min, 50%-0% B, 35-40 min, ...
1 CitationsSource
#1Lian-Xian GuoH-Index: 4
#2Xiao-Ming XuH-Index: 8
Last. Jiang-Hai WangH-Index: 14
view all 5 authors...
Ophiocordyceps sinensis is one rare medicinal fungus produced in the Qinghai-Tibetan Plateau. Its quality and price varies hugely with different habitat, and its numerous substitutes have sprung up in functional food markets. This paper aims to discriminate the geographic origin of wild O. sinensis and its substitutes via element analyzer–isotope ratio mass spectrometry and gas chromatography–isotope ratio mass spectrometry. The δ13C values of major fatty acids in the lipids of O. sinensis are c...
2 CitationsSource
#1Li ZhouH-Index: 2
#1Li Zhou (Guangdong Pharmaceutical University)H-Index: 1
Last. Lan-Ping GuoH-Index: 12
view all 7 authors...
The contents of five heavy metals (Cu, Pb, As, Cd, Hg) in 17 batches of Cordyceps sinensis were determined by microwave digestion-ICP-MS, and their distribution in C. sinensis were analyzed. The results showed that the contents of Cu, Pb, Cd and Hg in all batches were in accordance with the international standards of Chinese Medicine-Chinese Herbal Medicine Heavy Metal Limit, with their contents in the stroma higher than that in the caterpillar body, and the excess rate of As, which mainly conce...
5 CitationsSource
#2Parastoo Morteza-zadeh (UT: University of Tehran)H-Index: 1
Last. Ali GorjiH-Index: 30
view all 4 authors...
Objective: Arsenic, an environmental pollutant, decreases neuronal migration as well as cellular maturation and inhibits the proliferation of neural progenitor cells. Curcumin has been described as an antioxidant and neuroprotective agent with strong therapeutic potential in some neurological disorders. Human adipose-derived stem cells (hADSCs), a source of multipotent stem cells, can self-renew and differentiate into neural cells. The aim of the present study was to investigate the preventive e...
13 CitationsSource
#1Mohamed A. Salem (Cairo University)H-Index: 8
#2Michal Bernach (MPG: Max Planck Society)H-Index: 1
Last. Patrick Giavalisco (MPG: Max Planck Society)H-Index: 40
view all 4 authors...
Understanding of complex biological systems requires the measurement, analysis and integration of multiple compound classes of the living cell, usually determined by transcriptomic, proteomic, metabolomics and lipidomic measurements. In this protocol, we introduce a simple method for the reproducible extraction of metabolites, lipids and proteins from biological tissues using a single aliquot per sample. The extraction method is based on a methyl tert-butyl ether: methanol: water system for liqu...
8 CitationsSource
#1Michael Woolman (U of T: University of Toronto)H-Index: 5
#2Adam Gribble (U of T: University of Toronto)H-Index: 7
Last. Arash Zarrine-AfsarH-Index: 19
view all 11 authors...
Spatially Targeted Mass Spectrometry (MS) analysis using survey scans with an imaging modality often requires consecutive tissue slices, because of the tissue damage during survey scan or due to incompatible sample preparation requirements between the survey modality and MS. We report two spatially targeted MS analysis workflows based on polarized light imaging guidance that use the same tissue sample for survey and targeted analysis. The first workflow is applicable for thin-slice analysis, and...
14 CitationsSource
#1Niu J. Tan (UPM: Universiti Putra Malaysia)H-Index: 1
#2Leona Daniela Jeffery Daim (UPM: Universiti Putra Malaysia)H-Index: 4
Last. Karuppiah Thilakavathy (UPM: Universiti Putra Malaysia)H-Index: 5
view all 5 authors...
Effective protein extraction is essential especially in producing a well-resolved proteome on 2D-gels. A well-resolved placental cotyledon proteome, with good reproducibility, have allowed researchers to study the proteins underlying the physiology and pathophysiology of pregnancy. The aim of this study is to determine the best protein extraction protocol for the extraction of protein from placental cotyledons tissues for a two-dimensional gel electrophoresis (2D-GE). Based on widely used protei...
2 CitationsSource
Cited By6
#1Li-Qun Jin (Zhejiang University of Technology)H-Index: 9
#2Zhe-Wen Xu (Zhejiang University of Technology)H-Index: 1
Last. Yu-Guo Zheng (Zhejiang University of Technology)H-Index: 29
view all 6 authors...
OBJECTIVE To explore the optimal methods for the protoplast preparation and regeneration of Hirsutella sinensis by optimizing the limiting factors. RESULTS During the treatment of enzymatic protoplast preparation, mycelium cultured for 7 days was the optimal start material. The maximum protoplast preparation rate of 4.3 × 107 protoplasts/g fresh weight (FW) was obtained after 0.5 h treatment of 1 mg/ml mixed lytic enzymes in KH2PO4-K2HPO4 buffer (pH 5.5) with 0.6 M KCl at 18 °C. As for the proto...
#1Yujue Zhou (Sichuan Agricultural University)H-Index: 1
#2Min Wang (Sichuan Agricultural University)
Last. Jun Ma (Sichuan Agricultural University)H-Index: 4
view all 5 authors...
Natural Cordyceps sinensis, which is a valuable anti-tumor, immunomodulatory, and antiviral agent in Asia, has been overexploited in recent years. Therefore, it is important for cultivated C. sinensis to be recognized in the market. In this research, the main components of entirely cultivated, naturally grown C. sinensis, and stiff worms across different sampling years were detected and compared by HPLC-MS and UV spectrometry. The results indicated that the mean levels of adenosine and cordycepi...
#1Wei Jia (Shaanxi University of Science and Technology)H-Index: 8
#2Lin Shi (Shaanxi University of Science and Technology)H-Index: 4
Last. Xiaogang Chu (Shaanxi University of Science and Technology)H-Index: 11
view all 5 authors...
Abstract Cordyceps sinensis is a traditional Chinese food. A high-throughput screening and quantitative method based on hydrophilic interaction chromatography coupled to quadrupole/Orbitrap high resolution mass spectrometry has been proposed for the joint measurement of nucleosides and their corresponding nucleotides in thirty-two Cordyceps sinensis samples. A total of thirty-two natural samples from six regions were enrolled. A multiple-step analysis strategy, including features extraction, noi...
1 CitationsSource
#1Changliang Yao (CAS: Chinese Academy of Sciences)H-Index: 13
Last. Guo Dean (CAS: Chinese Academy of Sciences)H-Index: 2
view all 9 authors...
The term “Proteomics” refers to the characterization of the proteome, that is, all proteins present in a biological system [...]
#1Lian-Xian GuoH-Index: 4
#2Gui-Wei ZhangH-Index: 1
Last. Jiang-Hai WangH-Index: 14
view all 5 authors...
Ophiocordyceps sinensis has been utilized in China and adjacent countries for thousands of years as a rare functional food to promote health and treat diverse chronic diseases. In recent years, adulterants are usually identified in the processed products of wild O. sinensis. However, the effective adulteration examination has to be additionally performed except their routine test, and accordingly is time- and money-consuming. Recently, arsenic determination has become a necessary test for confir...
2 CitationsSource