Gain‐of‐function analysis of cis‐acting diversification elements in DT40 cells

Activation‐induced cytidine deaminase ( AID ) is required for the immunoglobulin diversification processes of somatic hypermutation, gene conversion and class‐switch recombination. The targeting of AID 's deamination activity is thought to be a combination of cis‐ and trans‐acting elements, but has not been fully elucidated. Deletion analysis of putative proximal cis‐regulatory motifs, while helpful, fails to identify additive versus cumulative...