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Published on Dec 1, 2017in BMC Veterinary Research 1.79
Yunyun Geng2
Estimated H-index: 2
(Hebei Normal University),
Jianchang Wang1
Estimated H-index: 1
+ 3 AuthorsYan-Zhong Chang1
Estimated H-index: 1
(Hebei Normal University)
Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was developed to detect PCV-2 using primers and an exo probe specific for the ORF2 gene. The reaction process can be completed in 20 min at 38 °C. The assay only detects PCV-2, as there was no cross-reaction with other pathogens important in pigs. Using the PCV-2 genomic DNA as template, the analytical sensiti...
Published on May 1, 2017in Archives of Virology 2.26
Soulasack Vannamahaxay1
Estimated H-index: 1
(CMU: Chiang Mai University),
Souliya Vongkhamchanh1
Estimated H-index: 1
(National University of Laos)
+ 4 AuthorsPhongsakorn Chuammitri2
Estimated H-index: 2
(CMU: Chiang Mai University)
The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of dogs were CPV positive by PCR. The partial VP2 gene was sequenced in 94 of the positive samples; 91 samples belonged to CPV-2c (426Glu) subtype, while 3 samples belonged to the CPV-2a...
Published on Apr 1, 2017in CSH Protocols
Michael R. Green5
Estimated H-index: 5
Joseph Sambrook29
Estimated H-index: 29
Published on Mar 31, 2017in PLOS ONE 2.78
Pei Zhou2
Estimated H-index: 2
(SCAU: South China Agricultural University),
Weijie Zeng2
Estimated H-index: 2
(SCAU: South China Agricultural University)
+ 1 AuthorsShoujun Li14
Estimated H-index: 14
(SCAU: South China Agricultural University)
To trace the evolution process of CPV-2, all of the VP2 gene sequences of CPV-2 and FPV (from 1978 to 2015) from GenBank were analyzed in this study. Then, several new ideas regarding CPV-2 evolution were presented. First, the VP2 amino acid 555 and 375 positions of CPV-2 were first ruled out as a universal mutation site in CPV-2a and amino acid 101 position of FPV feature I or T instead of only I in existing rule. Second, the recently confusing nomenclature of CPV-2 variants was substituted wit...
Published on Mar 1, 2017in Veterinary Microbiology 2.79
Yeison Duque-García1
Estimated H-index: 1
(UdeA: University of Antioquia),
Manuela Echeverri-Zuluaga1
Estimated H-index: 1
(UdeA: University of Antioquia)
+ 1 AuthorsJulian Ruiz-Saenz6
Estimated H-index: 6
(Grupo México)
Abstract Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae . Currently, there are three described antigenic variants (CPV-2a, CPV-2b, and CPV-2c). CPV-2c is an emerging virus that is seen as a global health hazard. The objective of this work was to confirm the presence of CPV-2 in d...
Published on Jan 1, 2017in Journal of Veterinary Medical Science 0.91
Mirna Faz1
Estimated H-index: 1
(UAEM: Universidad Autónoma del Estado de México),
José Simón Martínez1
Estimated H-index: 1
(UAEM: Universidad Autónoma del Estado de México)
+ 1 AuthorsRaúl Fajardo6
Estimated H-index: 6
(UAEM: Universidad Autónoma del Estado de México)
Published on Dec 1, 2016in Virology Journal 2.46
Shu-Yun Chiang1
Estimated H-index: 1
(NPUST: National Pingtung University of Science and Technology),
Hung-Yi Wu2
Estimated H-index: 2
(NCHU: National Chung Hsing University)
+ 2 AuthorsChao-Nan Lin2
Estimated H-index: 2
(NPUST: National Pingtung University of Science and Technology)
Background Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan.
Published on Nov 1, 2016
Ternenge Thaddaeus Apaa1
Estimated H-index: 1
(University of Agriculture, Makurdi),
Janet M. Daly29
Estimated H-index: 29
(University of Nottingham),
Rachael E. Tarlinton10
Estimated H-index: 10
(University of Nottingham)
Canine parvovirus type 2 (CPV-2) is a highly contagious viral disease with three variants (CPV-2a, CPV-2b and CPV-2c) currently circulating in dogs worldwide. The main aim of this study was to determine the prevalent CPV-2 variant in faecal samples from 53 dogs presenting with acute gastroenteritis suspected to be and consistent with CPV-2 to Nigerian Veterinary Clinics in 2013–2014. Seventy-five per cent of these dogs tested positive for CPV-2 in a commercial antigen test and/or by PCR. Partial...
Published on Sep 1, 2016in Veterinary World
Shalini Sharma2
Estimated H-index: 2
(College of Veterinary and Animal Sciences, Mannuthy),
Prasenjit Dhar2
Estimated H-index: 2
(College of Veterinary and Animal Sciences, Mannuthy)
+ 2 AuthorsMandeep Sharma8
Estimated H-index: 8
(College of Veterinary and Animal Sciences, Mannuthy)
Aim: The present study was conducted to detect the presence of canine parvovirus (CPV) among diarrheic dogs in Himachal Pradesh and to identify the most prevalent antigenic variant of CPV based on molecular typing and sequence analysis of VP2 gene.
Published on Jul 1, 2016in Molecular Biology and Evolution 14.80
Sudhir Kumar63
Estimated H-index: 63
(TU: Temple University),
Glen Stecher6
Estimated H-index: 6
(TU: Temple University),
Koichiro Tamura28
Estimated H-index: 28
(Tokyo Metropolitan University)
Abstract We present the latest version of the Molecular Evolutionary Genetics Analysis (Mega) software, which contains many sophisticated methods and tools for phylogenomics and phylomedicine. In this major upgrade, Mega has been optimized for use on 64-bit computing systems for analyzing larger datasets. Researchers can now explore and analyze tens of thousands of sequences in Mega The new version also provides an advanced wizard for building timetrees and includes a new functionality to automa...
Cited By2
Published on Jan 17, 2019in BMC Veterinary Research 1.79
Linlin Zhuang1
Estimated H-index: 1
(SEU: Southeast University),
Yongxin Ji + 4 AuthorsYu Zhang (SEU: Southeast University)
Background Canine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs. In recent years, magnetic separation has become an efficient and useful tool for bioassays. In this study, polymerase chain reaction (PCR) combined with fluorescent lateral flow immunoassay (LFIA) based on magnetic purification assay was developed for the quantitative detection of CPV-2.
Published on Apr 17, 2019in Journal of Virology 4.32
Olga Calatayud1
Estimated H-index: 1
Fernando Esperón12
Estimated H-index: 12
+ 6 AuthorsFelix Lankester13
Estimated H-index: 13
Carnivore parvoviruses infect wild and domestic carnivores and cross- species transmission is believed to occur. However, viral dynamics are not well understood nor the consequences to wild carnivore populations of the introduction of new strains into wild ecosystems. To clarify the ecology of these viruses in a multi-host system such as the Serengeti ecosystem and identify potential threats for wildlife conservation we analyzed, through real-time PCR, 152 samples belonging to 14 wild carnivore ...