Genotyping genome‐edited mutations in plants using CRISPR ribonucleoprotein complexes
Abstract
Summary Despite the great achievements in genome editing, accurately detecting mutations induced by sequence‐specific nucleases is still a challenge in plants, especially in polyploidy plants. An efficient detection method is particularly vital when the mutation frequency is low or when a large population needs to be screened. Here, we applied purified CRISPR ribonucleoprotein complexes to cleave PCR products for genome‐edited mutation detection...
Paper Details
Title
Genotyping genome‐edited mutations in plants using CRISPR ribonucleoprotein complexes
Published Date
May 29, 2018
Journal
Volume
16
Issue
12
Pages
2053 - 2062
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Notes
History