Characterizing metal-binding sites in proteins with X-ray crystallography

This protocol describes the guidelines and best practices for characterizing metal-binding sites in proteins through X-ray crystallography. Metals have crucial roles in many physiological, pathological, toxicological, pharmaceutical, and diagnostic processes. Proper handling of metal-containing macromolecule samples for structural studies is not trivial, and failure to handle them properly is often a source of irreproducibility caused by issues...