Rapid and Visual Detection ofCoxiella burnetiiUsing Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Coxiella burnetii , a global-distributed biological warfare agent, is the causative agent of Q fever. Correct diagnosis of Q fever is challenging and developing a fast, simple, and reliable detection method is necessary. In this study, recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) test was developed targeting 23S rRNA gene of C. burnetii Xinqiao strain. Primers and probe were designed and synthesized, with one...