Sequencing from Compomers: Using Mass Spectrometry for DNAde novoSequencing of 200+ nt

One of the main endeavors in today's life science remains the efficient sequencing of long DNA molecules. Today, most de novo sequencing of DNA is still performed using the electrophoresis-based Sanger concept of 1977, in spite of certain restrictions of this method. Methods using mass spectrometry to acquire the Sanger sequencing data are limited by short sequencing lengths of 15–25 nt. We propose a new method for DNA sequencing using...